Brand  (β version)

PDB ID: 1KJ8

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Ligands
Code Name Style Show Link
ATP Adenosine-5'-triphosphate PoSSuM
CL Chloride ion PoSSuM
EDO 1,2-ethanediol PoSSuM
GAR Glycinamide ribonucleotide PoSSuM
MG Magnesium ion PoSSuM
MPO 3[N-morpholino]propane sulfonic acid PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
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Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 1KJ8   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal Structure of PurT-Encoded Glycinamide Ribonucleotide Transformylase in Complex with Mg-ATP and GAR
Release Data : 2002-06-28
Compound :
mol_id molecule chains synonym
1 phosphoribosylglycinamide formyltransferase 2 A,B GART 2; GAR TRANSFORMYLASE 2; 5'-PHOSPHORIBOSYLGLYCINAMIDE
ec: 2.1.2.-
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: PURT
expression_system_strain: BL21(DE3)pLysS
expression_system_vector_type: PLASMID
expression_system_plasmid: pET-22b
Authors : Thoden, J.B., Firestine, S.M., Benkovic, S.J., Holden, H.M.
Keywords : ATP-grasp, purine biosynthesis, nucleotide, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.60 Å )
Citation :

PurT-encoded glycinamide ribonucleotide transformylase. Accommodation of adenosine nucleotide analogs within the active site.

Thoden, J.B.,Firestine, S.M.,Benkovic, S.J.  et al.
(2002)  J.Biol.Chem.  277 : 23898 - 23908

PubMed: 11953435
DOI: 10.1074/jbc.M202251200

Reaction

Chain : A, B
UniProt : P33221 (PURT_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
ATP + formate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = ADP + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate 6.3.1.21 HAMAP-Rule:MF_01643, PubMed:8117714, PubMed:8501063, PubMed:9184151
left-to-right
acetate + ATP = acetyl phosphate + ADP - PubMed:8117714, PubMed:9184151
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ATP + carbamate = ADP + carbamoyl phosphate - PubMed:9184151
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