Brand  (β version)

PDB ID: 1KEE

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Ligands
Code Name Style Show Link
MN Manganese (II) ion PoSSuM
K Potassium ion PoSSuM
CL Chloride ion PoSSuM
PO4 Phosphate ion PoSSuM
ADP Adenosine-5'-diphosphate PoSSuM
ORN L-ornithine PoSSuM
NET Tetraethylammonium ion PoSSuM
Non-standard Residues
Code Name Show
143 S-2,3-dihydro-5-glycin-2-yl-isoxazol-3-yl-cysteine
Glycosylation
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Structure summary

Code : 1KEE   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : Inactivation of the Amidotransferase Activity of Carbamoyl Phosphate Synthetase by the Antibiotic Acivicin
Release Data : 2001-12-21
Compound :
mol_id molecule chains synonym
1 Carbamoyl-phosphate synthetase large chain A,C,E,G CARBAMOYL-PHOSPHATE SYNTHETASE AMMONIA CHAIN
ec: 6.3.5.5
mol_id molecule chains synonym
2 Carbamoyl-phosphate synthetase small chain B,D,F,H CARBAMOYL-PHOSPHATE SYNTHETASE GLUTAMINE CHAIN
ec: 6.3.5.5
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: CARB
expression_system_strain: BL21DE3(pLysS)
expression_system_vector_type: plasmid
mol_id organism_scientific expression_system
2 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: CARA
expression_system_strain: BL21DE3(PysS)lysS)
expression_system_vector_type: plasmid
Authors : Miles, B.W., Thoden, J.B., Holden, H.M., Raushel, F.M.
Keywords : ATP grasp, channeling, antibiotic, LIGASE
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Inactivation of the amidotransferase activity of carbamoyl phosphate synthetase by the antibiotic acivicin.

Miles, B.W.,Thoden, J.B.,Holden, H.M.  et al.
(2002)  J.Biol.Chem.  277 : 4368 - 4373

PubMed: 11729189
DOI: 10.1074/jbc.M108582200

Reaction

Chain : A, C, E, G
UniProt : P00968 (CARB_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate 6.3.5.5 HAMAP- Rule:MF_01210, PubMed:4944634
-
[Carbamoyl phosphate synthase large chain]
2 ATP + hydrogencarbonate + NH4(+) = 2 ADP + carbamoyl phosphate + 2 H(+) + phosphate 		6.3.4.16 HAMAP- Rule:MF_01210, PubMed:4944634
-
Chain : B, D, F, H
UniProt : P00907 (CARA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate 6.3.5.5 HAMAP- Rule:MF_01209, PubMed:4944634
-
[Carbamoyl phosphate synthase small chain]
H2O + L-glutamine = L-glutamate + NH4(+) 		- HAMAP- Rule:MF_01209, PubMed:4944634
-