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Ligands
Code Name Style Show Link
EDO 1,2-ethanediol
MG Magnesium ion
Non-standard Residues
Code Name Show
OCS Cysteinesulfonic acid
Glycosylation
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Code : 1KCZ   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : Crystal Structure of beta-methylaspartase from Clostridium tetanomorphum. Mg-complex.
Release Data : 2001-12-19
Compound :
mol_id molecule chains synonym
1 beta-methylaspartase A,B METHYLASPARTATE AMMONIA-LYASE
ec: 4.3.1.2
Source :
mol_id organism_scientific expression_system
1 Clostridium tetanomorphum  (taxid:1553) Escherichia coli  (taxid:562)
expression_system_strain: BL21(lambda DE3)pLysS
expression_system_vector_type: plasmid
expression_system_plasmid: pJGetita
Authors : Asuncion, M., Blankenfeldt, W., Barlow, J.N., Gani, D., Naismith, J.H.
Keywords : beta zigzag, alpha/beta-barrel, LYASE
Exp. method : X-RAY DIFFRACTION ( 1.90 Å )
Citation :

The structure of 3-methylaspartase from Clostridium tetanomorphum functions via the common enolase chemical step.

Asuncion, M.,Blankenfeldt, W.,Barlow, J.N.  et al.
(2002)  J.Biol.Chem.  277 : 8306 - 8311

PubMed: 11748244
DOI: 10.1074/jbc.M111180200

Overexpression, purification, crystallization and data collection of 3-methylaspartase from Clostridium tetanomorphum

Asuncion, M.,Barlow, J.N.,Pollard, J.  et al.
(2001)  Acta Crystallogr.,Sect.D  57 : 731 - 733

DOI: 10.1107/S0907444901003225

Chain : A, B
UniProt : Q05514 (MAAL_CLOTT)
Reaction: EC: Evidence:
Physiological Direction:
(2S,3S)-3-methyl-L-aspartate = mesaconate + NH4(+) 4.3.1.2 PubMed:13630903, PubMed:1420191, PubMed:19670200, PubMed:22614383
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