Brand  (β version)

PDB ID: 1KCE

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Ligands
Code Name Style Show Link
CB3 10-propargyl-5,8-dideazafolic acid PoSSuM
UMP 2'-deoxyuridine 5'-monophosphate PoSSuM
Non-standard Residues
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CXM N-carboxymethionine
Glycosylation
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Structure summary

Code : 1KCE   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : E. COLI THYMIDYLATE SYNTHASE MUTANT E58Q IN COMPLEX WITH CB3717 AND 2'-DEOXYURIDINE 5'-MONOPHOSPHATE (DUMP)
Release Data : 1997-04-21
Compound :
mol_id molecule chains
1 THYMIDYLATE SYNTHASE A,B
ec: 2.1.1.45
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
cell_line: X2913
gene: THYA
expression_system_plasmid: PTHYA-WT
expression_system_gene: THYA
Authors : Sage, C.R., Rutenber, E.E., Stout, T.J., Stroud, R.M.
Keywords : TRANSFERASE, REACTION INTERMEDIATE
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

An essential role for water in an enzyme reaction mechanism: the crystal structure of the thymidylate synthase mutant E58Q.

Sage, C.R.,Rutenber, E.E.,Stout, T.J.  et al.
(1996)  Biochemistry  35 : 16270 - 16281

PubMed: 8973201
DOI: 10.1021/bi961269r

Structure, Multiple Site Binding, and Segmental Accommodation in Thymidylate Synthase on Binding Dump and an Anti-Folate

Montfort, W.R.,Perry, K.M.,Fauman, E.B.  et al.
(1990)  Biochemistry  29 : 6964

Erratum. Structure, Multiple Site Binding, and Segmental Accommodation in Thymidylate Synthase on Binding Dump and an Anti-Folate

Montfort, W.R.,Perry, K.M.,Fauman, E.B.  et al.
(1990)  Biochemistry  29 : 10864

Reaction

Chain : A, B
UniProt : P0A884 (TYSY_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8- dihydrofolate + dTMP 2.1.1.45 HAMAP-Rule:MF_00008, PubMed:2223754, PubMed:3286637, PubMed:9826509
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