Brand  (β version)

PDB ID: 1K8W

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Ligands
Code Name Style Show Link
SO4 Sulfate ion PoSSuM
Non-standard Residues
Code Name Show
FHU (5s,6r)-5-fluoro-6-hydroxy-pseudouridine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1K8W   PDBj   RCSB PDB   PDBe
Header : LYASE/RNA
Title : Crystal structure of the E. coli pseudouridine synthase TruB bound to a T stem-loop RNA
Release Data : 2001-12-31
Compound :
mol_id molecule chains
1 5'-R(*GP*GP*CP*AP*AP*CP*GP*GP*UP*(FHU)P*CP*GP*AP*UP*CP*CP*CP*GP*UP*UP*GP*C)-3' B
other_details: T stem-loop RNA
mol_id molecule chains synonym
2 tRNA Pseudouridine Synthase B A TruB Pseudouridine Synthase, tRNA Pseudouridine 55 Synthase, Pseudouridylate synthase
ec: 4.2.1.70
fragment: Residues 10-314
mutation: Residues 1-9 replaced with a His-Tag
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific expression_system
2 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
Authors : Hoang, C., Ferre-D'Amare, A.R.
Keywords : protein-RNA complex, T stem-loop, tRNA, LYASE-RNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 1.85 Å )
Citation :

Cocrystal structure of a tRNA Psi55 pseudouridine synthase: nucleotide flipping by an RNA-modifying enzyme.

Hoang, C.,Ferre-D'Amare, A.R.
(2001)  Cell(Cambridge,Mass.)  107 : 929 - 939

PubMed: 11779468
DOI: 10.1016/S0092-8674(01)00618-3

Reaction

Chain : A
UniProt : P60340 (TRUB_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
uridine(55) in tRNA = pseudouridine(55) in tRNA 5.4.99.25 HAMAP-Rule:MF_01080, PubMed:7489483
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