Brand  (β version)

PDB ID: 1JZ3

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
BTB 2-[bis-(2-hydroxy-ethyl)-amino]-2-hydroxymethyl-propane-1,3-diol PoSSuM
DMS Dimethyl sulfoxide PoSSuM
MG Magnesium ion PoSSuM
NA Sodium ion PoSSuM
2DG 2-deoxy-alpha-D-galactopyranose PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1JZ3   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : E. COLI (lacZ) BETA-GALACTOSIDASE-TRAPPED 2-DEOXY-GALACTOSYL ENZYME INTERMEDIATE
Release Data : 2001-12-07
Compound :
mol_id molecule chains synonym
1 Beta-Galactosidase A,B,C,D LACTASE; lacZ; beta-d-galactosidase
ec: 3.2.1.23
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: lacZ
expression_system_strain: JM109
expression_system_vector_type: PLASMID
expression_system_plasmid: pET15B
Authors : Juers, D.H., Matthews, B.W.
Keywords : TIM BARREL (ALPHA/BETA BARREL), JELLY-ROLL BARREL, IMMUNOGLOBULIN, BETA SUPERSANDWICH, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.75 Å )
Citation :

A Structural View of the Action of Escherichia Coli (Lacz) Beta-Galactosidase

Juers, D.H.,Heightman, T.D.,Vasella, A.  et al.
(2001)  Biochemistry  40 : 14781 - 14794

PubMed: 11732897
DOI: 10.1021/bi011727i

High Resolution Structure of Beta-Galactosidase in a New Crystal Form Reveals Multiple Metal-Binding Sites and Provides a Structural Basis for Alpha-Complementation

Juers, D.H.,Jacobson, R.H.,Wigley, D.  et al.
(2000)  Protein Sci.  9 : 1685 - 1699

Structural Comparisons of Tim Barrel Proteins Suggest Functional and Evolutionary Relationships between Beta-Galactosidase and Other Glycohydrolases

Juers, D.H.,Huber, R.E.,Matthews, B.W.
(1999)  Protein Sci.  8 : 122 - 136

Three-Dimensional Structure of Beta-Galactosidase from E. Coli

Jacobson, R.H.,Zhang, X.J.,Dubose, R.F.  et al.
(1994)  Nature  369 : 761 - 766

DOI: 10.1038/369761a0

Crystallization of beta-galactosidase from Escherichia coli

Jacobson, R.H.,Matthews, B.W.
(1992)  J.Mol.Biol.  223 : 1177 - 1182

Reaction

Chain : A, B, C, D
UniProt : P00722 (BGAL_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. 3.2.1.23 -
-