Brand  (β version)

PDB ID: 1JWJ

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Ligands
Code Name Style Show Link
HEM Protoporphyrin ix containing Fe PoSSuM
H4B 5,6,7,8-tetrahydrobiopterin PoSSuM
GOL Glycerol PoSSuM
BOG Octyl beta-D-glucopyranoside PoSSuM
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Structure summary

Code : 1JWJ   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Murine Inducible Nitric Oxide Synthase Oxygenase Dimer (Delta 65) with W457F Mutation at Tetrahydrobiopterin Binding Site
Release Data : 2001-10-31
Compound :
mol_id molecule chains synonym
1 Nitric Oxide Synthase A,B NOS, type II, Inducible NOS, INOS, Macrophage NOS, MAC-NOS
ec: 1.14.13.39
fragment: Oxygenase Domain 65-498
mutation: YES
Source :
mol_id organism_scientific organism_common expression_system
1 Mus musculus  (taxid:10090) House mouse Escherichia coli  (taxid:562)
expression_system_vector_type: PLASMID
expression_system_plasmid: pCWori
Authors : Aoyagi, M., Arvai, A.S., Ghosh, S., Stuehr, D.J., Tainer, J.A., Getzoff, E.D.
Keywords : Nitric Oxide L-Arginine Monooxygenase, Heme, Dimer, NOS, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.60 Å )
Citation :

Structures of tetrahydrobiopterin binding-site mutants of inducible nitric oxide synthase oxygenase dimer and implicated roles of Trp457.

Aoyagi, M.,Arvai, A.S.,Ghosh, S.  et al.
(2001)  Biochemistry  40 : 12826 - 12832

PubMed: 11669619
DOI: 10.1021/bi011183k

A Conserved Tryptophan in Nitric Oxide Synthase Regulates Heme-dioxy Reduction by Tetrahydrobiopterin

Wang, Z.-Q.,Wei, C.-C.,Ghosh, S.  et al.
(2001)  Biochemistry  40 : 12819 - 12825

DOI: 10.1021/bi011182s

Reaction

Chain : A, B
UniProt : P29477 (NOS2_MOUSE)
Reaction: EC: Evidence:
Physiological Direction:
H(+) + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline + 3 NADP(+) + 2 nitric oxide 1.14.13.39 UniProtKB:P35228
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