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Ligands
Code Name Style Show Link
CEN 7beta-(4carboxybutanamido) cephalosporanic acid
Non-standard Residues
Code Name Show
MSE Selenomethionine
Glycosylation
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Code : 1JVZ   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid
Release Data : 2002-09-01
Compound :
mol_id molecule chains synonym
1 cephalosporin acylase alpha chain A glutaryl 7-aminocephalosporanic acid acylase
fragment: RESIDUES 30-187
mol_id molecule chains synonym
2 cephalosporin acylase beta chain B glutaryl 7-aminocephalosporanic acid acylase
fragment: RESIDUES 199-718
Source :
mol_id organism_scientific expression_system
1 Brevundimonas diminuta  (taxid:293) Escherichia coli  (taxid:562)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET24d(+)
mol_id organism_scientific expression_system
2 Brevundimonas diminuta  (taxid:293) Escherichia coli  (taxid:562)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET24d(+)
Authors : Kim, Y., Hol, W.G.J.
Keywords : cephalosporin acylase, glutaryl-7-aminocephalosporanic acid, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.6 Å )
Citation :

Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity

Kim, Y.,Hol, W.G.
(2001)  CHEM.BIOL.  8 : 1253 - 1264

PubMed: 11755403
DOI: 10.1016/S1074-5521(01)00092-8

Chain : B
UniProt : Q9L5D6 (G7AC_BREDI)
Reaction: EC: Evidence:
Physiological Direction:
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O = (7R)-7- aminocephalosporanate + glutarate 3.5.1.93 -
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