Brand  (β version)

PDB ID: 1JSC

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Ligands
Code Name Style Show Link
2HP Dihydrogenphosphate ion PoSSuM
FAD Flavin-adenine dinucleotide PoSSuM
K Potassium ion PoSSuM
MG Magnesium ion PoSSuM
TPP Thiamine diphosphate PoSSuM
Non-standard Residues
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Structure summary

Code : 1JSC   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : Crystal Structure of the Catalytic Subunit of Yeast Acetohydroxyacid Synthase: A target for Herbicidal Inhibitors
Release Data : 2002-01-16
Compound :
mol_id molecule chains synonym
1 ACETOHYDROXY-ACID SYNTHASE A,B ACETOLACTATE SYNTHASE
ec: 4.1.3.18
fragment: MATURE CATALYTIC SUBUNIT
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharomyces cerevisiae  (taxid:4932) Baker's yeast Escherichia coli BL21(DE3)  (taxid:469008)
gene: ILV2
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET30C
Authors : Pang, S.S., Duggleby, R.G., Guddat, L.W.
Keywords : ACETOHYDROXYACID SYNTHASE, ACETOLACTATE SYNTHASE, FAD, THIAMIN DIPHOSPHATE, HERBICIDE INHIBITION, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.6 Å )
Citation :

Crystal structure of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors.

Pang, S.S.,Duggleby, R.G.,Guddat, L.W.
(2002)  J.Mol.Biol.  317 : 249 - 262

PubMed: 11902841
DOI: 10.1006/jmbi.2001.5419

Reaction

Chain : A, B
UniProt : P07342 (ILVB_YEAST)
Reaction: EC: Evidence:
Physiological Direction:
2 pyruvate + H(+) = (2S)-2-acetolactate + CO2 2.2.1.6 PubMed:10213630
left-to-right
2-oxobutanoate + pyruvate + H(+) = (S)-2-ethyl-2-hydroxy-3- oxobutanoate + CO2 2.2.1.6 PubMed:10213630
left-to-right
Cofactor: Evidence: Note:
Mg(2+) ECO:0000250
Binds 1 Mg(2+) ion per subunit.
thiamine diphosphate ECO:0000250
Binds 1 thiamine pyrophosphate per subunit.