Brand  (β version)

PDB ID: 1JOM

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
CL Chloride ion PoSSuM
EOH Ethanol PoSSuM
FFO N-[4-({[(6s)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid PoSSuM
Non-standard Residues
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Structure summary

Code : 1JOM   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : THE CRYSTAL STRUCTURE OF THE BINARY COMPLEX BETWEEN FOLINIC ACID (LEUCOVORIN) AND E. COLI DIHYDROFOLATE REDUCTASE
Release Data : 1996-11-08
Compound :
mol_id molecule chains synonym
1 DIHYDROFOLATE REDUCTASE A 5,6,7,8-TETRAHYDROFOLATE\: 2 NADP+ OXIDOREDUCTASE
ec: 1.5.1.3
other_details: IN BINARY COMPLEX WITH FOLINIC ACID (LEUCOVORIN)
Source :
mol_id organism_scientific
1 Escherichia coli  (taxid:562)
Authors : Reyes, V.M., Lee, H., Kraut, J.
Keywords : METHOTREXATE RESISTANCE, ONE-CARBON METABOLISM OXIDOREDUCTASE, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.90 Å )
Citation :

Crystal structures of Escherichia coli dihydrofolate reductase complexed with 5-formyltetrahydrofolate (folinic acid) in two space groups: evidence for enolization of pteridine O4.

Lee, H.,Reyes, V.M.,Kraut, J.
(1996)  Biochemistry  35 : 7012 - 7020

PubMed: 8679526
DOI: 10.1021/bi960028g

Isomorphous Crystal Structures of Escherichia Coli Dihydrofolate Reductase Complexed with Folate, 5-Deazafolate, and 5,10-Dideazatetrahydrofolate: Mechanistic Implications

Reyes, V.M.,Sawaya, M.R.,Brown, K.A.  et al.
(1995)  Biochemistry  34 : 2710

Crystal Structure of Unliganded Escherichia Coli Dihydrofolate Reductase. Ligand-Induced Conformational Changes and Cooperativity in Binding

Bystroff, C.,Kraut, J.
(1991)  Biochemistry  30 : 2227

Crystal Structures of Recombinant Human Dihydrofolate Reductase Complexed with Folate and 5-Deazafolate

Davies II, J.F.,Delcamp, T.J.,Prendergast, N.J.  et al.
(1990)  Biochemistry  29 : 9467

Crystal Structures of Escherichia Coli Dihydrofolate Reductase: The Nadp+ Holoenzyme and the Folate.Nadp+ Ternary Complex. Substrate Binding and a Model for the Transition State

Bystroff, C.,Oatley, S.J.,Kraut, J.
(1990)  Biochemistry  29 : 3263

Crystal Structures of Escherichia Coli and Lactobacillus Casei Dihydrofolate Reductase Refined at 1.7 A Resolution. II. Environment of Bound Nadph and Implications for Catalysis

Filman, D.J.,Bolin, J.T.,Matthews, D.A.  et al.
(1982)  J.Biol.Chem.  257 : 13663

Crystal Structures of Escherichia Coli and Lactobacillus Casei Dihydrofolate Reductase Refined at 1.7 A Resolution. I. General Features and Binding of Methotrexate

Bolin, J.T.,Filman, D.J.,Matthews, D.A.  et al.
(1982)  J.Biol.Chem.  257 : 13650

Reaction

Chain : A
UniProt : P0ABQ4 (DYR_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH 1.5.1.3 PROSITE- ProRule:PRU00660, PubMed:16510443
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