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Ligands
Code Name Style Show Link
FRU Beta-D-fructofuranose
GLC Alpha-D-glucopyranose
: Polysaccharide
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Code : 1JGI   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal Structure of the Active Site Mutant Glu328Gln of Amylosucrase from Neisseria polysaccharea in Complex with the Natural Substrate Sucrose
Release Data : 2001-10-31
Compound :
mol_id molecule chains
1 amylosucrase A
ec: 2.4.1.4
mutation: E328Q
Source :
mol_id organism_scientific expression_system
1 Neisseria polysaccharea  (taxid:489) Escherichia coli  (taxid:562)
Authors : Mirza, O., Skov, L.K., Gajhede, M.
Keywords : active site mutant Glu328Gln, sucrose complex, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Crystal structures of amylosucrase from Neisseria polysaccharea in complex with D-glucose and the active site mutant Glu328Gln in complex with the natural substrate sucrose.

Mirza, O.,Skov, L.K.,Remaud-Simeon, M.  et al.
(2001)  Biochemistry  40 : 9032 - 9039

PubMed: 11467966
DOI: 10.1021/bi010706l

Chain : A
UniProt : Q9ZEU2 (AMYS_NEIPO)
Reaction: EC: Evidence:
Physiological Direction:
[(1->4)-alpha-D-glucosyl](n) + sucrose = [(1->4)-alpha-D- glucosyl](n+1) + D-fructose 2.4.1.4 PubMed:10767427, PubMed:10828446
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