Brand  (β version)

PDB ID: 1J79

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Ligands
Code Name Style Show Link
NCD N-carbamoyl-L-aspartate PoSSuM
ORO Orotic acid PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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KCX Lysine nz-carboxylic acid
Glycosylation
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Structure summary

Code : 1J79   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Molecular Structure of Dihydroorotase: A Paradigm for Catalysis Through the Use of a Binuclear Metal Center
Release Data : 2001-06-20
Compound :
mol_id molecule chains synonym
1 dihydroorotase A,B dhoase
ec: 3.5.2.3
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: PYRC
Authors : Thoden, J.B., Phillips Jr., G.N., Neal, T.M., Raushel, F.M., Holden, H.M.
Keywords : TIM barrel, metalloenzyme, pyrimidine biosynthesis, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.70 Å )
Citation :

Molecular structure of dihydroorotase: a paradigm for catalysis through the use of a binuclear metal center.

Thoden, J.B.,Phillips Jr., G.N.,Neal, T.M.  et al.
(2001)  Biochemistry  40 : 6989 - 6997

PubMed: 11401542
DOI: 10.1021/bi010682i

Reaction

Chain : A, B
UniProt : P05020 (PYRC_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+) 3.5.2.3 HAMAP-Rule:MF_00219, PubMed:15610022, PubMed:6142052
-
Cofactor: Evidence: Note:
Zn(2+) ECO:0000255 | HAMAP-Rule:MF_00219
ECO:0000269 | PubMed:11401542
ECO:0000269 | PubMed:15610022
ECO:0000269 | PubMed:15826651
ECO:0000269 | PubMed:1671037
ECO:0000269 | PubMed:6142052
Binds 2 Zn(2+) ions per subunit (PubMed:11401542, PubMed:15826651, PubMed:6142052). In vitro, can also use Co(2+) or Cd(2+) (PubMed:15610022, PubMed:1671037).