Brand  (β version)

PDB ID: 1J3J

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Ligands
Code Name Style Show Link
CP6 5-(4-chloro-phenyl)-6-ethyl-pyrimidine-2,4-diamine PoSSuM
NDP Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate PoSSuM
UMP 2'-deoxyuridine 5'-monophosphate PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1J3J   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE, TRANSFERASE
Title : Double mutant (C59R+S108N) Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS) complexed with pyrimethamine, NADPH, and dUMP
Release Data : 2003-05-06
Compound :
mol_id molecule chains
1 Bifunctional dihydrofolate reductase-thymidylate synthase A,B
ec: 1.5.1.3, 2.1.1.45
fragment: RESIDUES 1-280
mutation: C59R, S108N
mol_id molecule chains
2 Bifunctional dihydrofolate reductase-thymidylate synthase C,D
ec: 1.5.1.3, 2.1.1.45
fragment: RESIDUES 281-608
Source :
mol_id organism_scientific organism_common expression_system
1 Plasmodium falciparum  (taxid:5833) Malaria parasite P. falciparum Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pLysS
mol_id organism_scientific organism_common expression_system
2 Plasmodium falciparum  (taxid:5833) Malaria parasite P. falciparum Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pLysS
Authors : Yuvaniyama, J., Chitnumsub, P., Kamchonwongpaisan, S., Vanichtanankul, J., Sirawaraporn, W., Taylor, P., Walkinshaw, M., Yuthavong, Y.
Keywords : bifunctional, OXIDOREDUCTASE, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Insights into antifolate resistance from malarial DHFR-TS structures.

Yuvaniyama, J.,Chitnumsub, P.,Kamchonwongpaisan, S.  et al.
(2003)  NAT.STRUCT.BIOL.  10 : 357 - 365

PubMed: 12704428
DOI: 10.1038/nsb921

Reaction

Chain : C, D
UniProt : P13922 (DRTS_PLAFK)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH 1.5.1.3 -
-
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8- dihydrofolate + dTMP 2.1.1.45 -
-