Brand  (β version)

PDB ID: 1IWJ

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Ligands
Code Name Style Show Link
CAM Camphor PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1IWJ   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Putidaredoxin-Binding Stablilizes an Active Conformer of Cytochrome P450cam in its Reduced State; Crystal Structure of Mutant(109K) Cytochrome P450cam
Release Data : 2002-06-05
Compound :
mol_id molecule chains
1 CYTOCHROME P450-CAM A
ec: 1.14.15.1
mutation: R109K
Source :
mol_id organism_scientific expression_system
1 Pseudomonas putida  (taxid:303) Escherichia coli  (taxid:562)
expression_system_strain: JM109
expression_system_vector_type: PLASMID
expression_system_plasmid: PUC19
Authors : Nagano, S., Shimada, H., Tarumi, A., Hishiki, T., Kimata-Ariga, Y., Egawa, T., Park, S.-Y., Adachi, S., Shiro, Y., Ishimura, Y., RIKEN Structural Genomics/Proteomics Initiative (RSGI)
Keywords : Putidaredoxin Binding site, Cytochrome P450cam, Mutant 109K, RIKEN Structural Genomics/Proteomics Initiative, RSGI, Structural Genomics, Oxidoreductase
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Infrared spectroscopic and mutational studies on putidaredoxin-induced conformational changes in ferrous CO-P450cam

Nagano, S.,Shimada, H.,Tarumi, A.  et al.
(2003)  Biochemistry  42 : 14507 - 14514

PubMed: 14661963
DOI: 10.1021/bi035410p

Reaction

Chain : A
UniProt : P00183 (CPXA_PSEPU)
Reaction: EC: Evidence:
Physiological Direction:
(1R,4R)-camphor + 2 H(+) + O2 + 2 reduced [2Fe-2S]- [putidaredoxin] = (1R,4R,5R)-5-hydroxycamphor + H2O + 2 oxidized [2Fe-2S]-[putidaredoxin] 1.14.15.1 -
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