Brand  (β version)

PDB ID: 1IG9

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
TTP Thymidine-5'-triphosphate PoSSuM
Non-standard Residues
Code Name Show
DOC 2',3'-dideoxycytidine-5'-monophosphate
Glycosylation
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Modification
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Structure summary

Code : 1IG9   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase
Release Data : 2001-06-11
Compound :
mol_id molecule chains
1 5'-D(*AP*CP*AP*GP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3' T
other_details: Substrate DNA, template strand
mol_id molecule chains
2 5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*CP*(DOC))-3' P
other_details: Substrate DNA, primer strand (dideoxy-terminated)
mol_id molecule chains
3 DNA POLYMERASE A
ec: 2.7.7.7
mutation: Exonuclease-deficient (D222A/D327A) mutant
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Enterobacteria phage RB69  (taxid:12353) Escherichia coli BL21(DE3)  (taxid:469008)
gene: gp43
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pRB.43 exo-
Authors : Franklin, M.C., Wang, J., Steitz, T.A.
Keywords : DNA polymerase, catalytic complex, fidelity, two metal ion mechanism, TRANSFERASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.60 Å )
Citation :

Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase

Franklin, M.C.,Wang, J.,Steitz, T.A.
(2001)  Cell(Cambridge,Mass.)  105 : 657 - 667

PubMed: 11389835
DOI: 10.1016/S0092-8674(01)00367-1

Crystal Structure of a Pol Alpha Family Replication DNA Polymerase from Bacteriophage RB69

Wang, J.,Sattar, A.K.,Wang, C.C.  et al.
(1997)  Cell(Cambridge,Mass.)  89 : 1087 - 1099

DOI: 10.1016/S0092-8674(00)80296-2

Building a Replisome from Interacting Pieces: Sliding Clamp Complexed to a Peptide from DNA Polymerase and a Polymerase Editing Complex

Shamoo, Y.,Steitz, T.A.
(1999)  Cell(Cambridge,Mass.)  99 : 155 - 166

DOI: 10.1016/S0092-8674(00)81647-5

Reaction

Chain : A
UniProt : Q38087 (DPOL_BPR69)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 HAMAP-Rule:MF_04100, PubMed:17098747, PubMed:18503083, PubMed:20795733, PubMed:22616982, PubMed:24116139
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000255 | HAMAP-Rule:MF_04100
ECO:0000269 | PubMed:20166752
ECO:0000269 | PubMed:22026756
ECO:0000305 | PubMed:11389835
ECO:0000305 | PubMed:15057282
ECO:0000305 | PubMed:15057283
ECO:0000305 | PubMed:17718515
ECO:0000305 | PubMed:18503083
ECO:0000305 | PubMed:20166748
ECO:0000305 | PubMed:21158418
ECO:0000305 | PubMed:21566148
ECO:0000305 | PubMed:21923197
ECO:0000305 | PubMed:22571765
ECO:0000305 | PubMed:23214497
ECO:0000305 | PubMed:23921641
ECO:0000305 | PubMed:24116139
-