Brand  (β version)

PDB ID: 1HXC

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Ligands
Code Name Style Show Link
FHP 1-hydroxy-3,7,11-trimethyldodeca-2,6,10-triene phosphonic acid PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1HXC   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : CRYSTAL STRUCTURE OF TEAS C440W
Release Data : 2003-06-24
Compound :
mol_id molecule chains synonym
1 5-EPI-ARISTOLOCHENE SYNTHASE A TEAS
ec: 4.1.99.7
mutation: C440W
Source :
mol_id organism_scientific organism_common expression_system
1 Nicotiana tabacum  (taxid:4097) Common tobacco Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_strain: BL21 (DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET28B(+)
Authors : Starks, C.S., Rising, K.A., Chappell, J., Noel, J.P.
Keywords : ISOPRENOID SYNTHASE, ISOPRENOID CYCLASE, 5-EPI-ARISTOLOCHENE SYNTHASE, ISOPRENOID BIOSYNTHESIS, NATURAL PRODUCTS BIOSYNTHESIS, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.25 Å )
Citation :

Single Active Site Mutations Change the Specificity of a Sesquiterpene Cyclase

Starks, C.S.,Rising, K.A.,Chappell, J.  et al.
To be Published 

Reaction

Chain : A
UniProt : Q40577 (5EAS_TOBAC)
Reaction: EC: Evidence:
Physiological Direction:
(2E,6E)-farnesyl diphosphate = (+)-5-epi-aristolochene + diphosphate 4.2.3.61 PubMed:16375847, PubMed:20175559, PubMed:25897591, PubMed:26378620, Ref.3
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(2Z,6E)-farnesyl diphosphate = (+)-2-epi-prezizaene + diphosphate 4.2.3.226 PubMed:20175559, PubMed:20201526
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(2Z,6E)-farnesyl diphosphate = (-)-alpha-cedrene + diphosphate 4.2.3.227 PubMed:20175559, PubMed:20201526
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(2Z,6E)-farnesyl diphosphate = (-)-beta-curcumene + diphosphate - PubMed:20175559, PubMed:20201526
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Cofactor: Evidence: Note:
Mg(2+) ECO:0000269 | PubMed:9295271
Binds 3 Mg(2+) ions per subunit.