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Ligands
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CMP Adenosine-3',5'-cyclic-monophosphate
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Code : 1HW5   PDBj   RCSB PDB   PDBe
Header : GENE REGULATION
Title : THE CAP/CRP VARIANT T127L/S128A
Release Data : 2001-01-17
Compound :
mol_id molecule chains synonym
1 CATABOLITE GENE ACTIVATOR A,B CAMP RECEPTOR PROTEIN
mutation: T127L, S128A
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli K12  (taxid:83333)
gene: CRP/CAP
expression_system_strain: K12
expression_system_vector_type: PLASMID
Authors : Chu, S.Y., Tordova, M., Gilliland, G.L., Gorshkova, I., Shi, Y.
Keywords : cAMP Receptor Protein, Catabolite Activator Protein (CAP) Transcription, Allostery, cAMP, cyclic AMP Mutant, GENE REGULATION
Exp. method : X-RAY DIFFRACTION ( 1.82 Å )
Citation :

The structure of the T127L/S128A mutant of cAMP receptor protein facilitates promoter site binding

Chu, S.Y.,Tordova, M.,Gilliland, G.L.  et al.
(2001)  J.Biol.Chem.  276 : 11230 - 11236

PubMed: 11124966
DOI: 10.1074/jbc.M010428200

The Structure of a CAP-DNA Complex Having Two cAMP Molecules Bound To Each Monomer

Passner, J.M.,Steitz, T.A.
(1997)  Proc.Natl.Acad.Sci.USA  94 : 2843 - 2847

DOI: 10.1073/pnas.94.7.2843

Structure of a Complex of Catabolite Gene Activator Protein and Cyclic AMP Refined at 2.5 A Resolution

Weber, I.T.,Steitz, T.A.
(1987)  J.Mol.Biol.  198 : 311 - 326

Crystal Structure of a Cyclic AMP-independent Mutant of Catabolite Gene Activator Protein

Weber, I.T.,Gilliland, G.L.,Harman, J.G.  et al.
(1987)  J.Biol.Chem.  262 : 5630 - 5636

Chain : A, B
UniProt : P0ACJ8 (CRP_ECOLI)
Reaction : -