Brand  (β version)

PDB ID: 1HUO

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Ligands
Code Name Style Show Link
CR Chromium ion PoSSuM
TTE Phosphomethyl phosphonic acid deoxythymidylate ester PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1HUO   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : CRYSTAL STRUCTURE OF DNA POLYMERASE BETA COMPLEXED WITH DNA AND CR-TMPPCP
Release Data : 2001-04-23
Compound :
mol_id molecule chains
1 5'-D(*AP*AP*TP*AP*GP*GP*CP*GP*TP*CP*G)-3' T,C
mol_id molecule chains
2 5'-D(P*CP*GP*AP*CP*GP*CP*C)-3' P,D
mol_id molecule chains
3 DNA POLYMERASE BETA A,B
ec: 2.7.7.7
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific organism_common expression_system
3 Rattus norvegicus  (taxid:10116) Norway rat Escherichia coli  (taxid:562)
expression_system_vector_type: PLASMID
expression_system_plasmid: PT17B
Authors : Arndt, J.W., Gong, W., Zhong, X., Showalter, A.K., Liu, J., Lin, Z., Paxson, C., Tsai, M.-D., Chan, M.K.
Keywords : PROTEIN-DNA COMPLEX, TRANSFERASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.6 Å )
Citation :

Insight into the catalytic mechanism of DNA polymerase beta: structures of intermediate complexes.

Arndt, J.W.,Gong, W.,Zhong, X.  et al.
(2001)  Biochemistry  40 : 5368 - 5375

PubMed: 11330999
DOI: 10.1021/bi002176j

Reaction

Chain : A, B
UniProt : P06766 (DPOLB_RAT)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 UniProtKB:P06746
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a 5'-end 2'-deoxyribose-2'-deoxyribonucleotide-DNA = (2E,4S)- 4-hydroxypenten-2-al-5-phosphate + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+) - UniProtKB:P06746
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2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+) 4.2.99.18 UniProtKB:P06746
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000250 | UniProtKB:P06746
Binds 2 magnesium ions per subunit.