Brand  (β version)

PDB ID: 1HF6

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
ACY Acetic acid PoSSuM
GOL Glycerol PoSSuM
SO4 Sulfate ion PoSSuM
BGC Beta-D-glucopyranose PoSSuM
GLC Alpha-D-glucopyranose PoSSuM
: Polysaccharide
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1HF6   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHAERENS IN THE ORTHORHOMBIC CRYSTAL FORM IN COMPLEX WITH CELLOTRIOSE
Release Data : 2001-11-29
Compound :
mol_id molecule chains synonym
1 ENDOGLUCANASE B A ENDO-1,4-BETA-GLUCANASE, CELLULASE SYNONYM: CELLULASE, ENDO-1,4-BETA-GLUCANASE
ec: 3.2.1.4
fragment: CATALYTIC CORE DOMAIN ONLY
Source :
mol_id organism_scientific expression_system
1 BACILLUS AGARADHAERENS  (taxid:76935) BACILLUS SUBTILIS  (taxid:1423)
expression_system_strain: PL2306
expression_system_vector_type: BACILLUS, CELLULASE NEGATIVE STRAIN
expression_system_vector: PMOL995
expression_system_plasmid: THERMAMYL-AMYLASE PROMOT
Authors : Varrot, A., Withers, S., Vasella, A., Schulein, M., Davies, G.J.
Keywords : HYDROLASE, CELLULOSE DEGRADATION, ENDOGLUCANASE, GLYCOSHYDROLASE FAMILY 5
Exp. method : X-RAY DIFFRACTION ( 1.15 Å )
Citation :

Direct Experimental Observation of the Hydrogen-Bonding Network of a Glycosidase Along its Reaction Coordinate Revealed by Atomic Resolution Analyses of Endoglucanase Cel5A

Varrot, A.,Davies, G.J.
(2003)  Acta Crystallogr.,Sect.D  59 : 447

PubMed: 12595701
DOI: 10.1107/S0907444902023405

Reaction

Chain : A
UniProt : O85465 (GUN5_SALAG)
Reaction: EC: Evidence:
Physiological Direction:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. 3.2.1.4 PubMed:9485319, PubMed:9718293
-