Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
PB Lead (II) ion
Code : 1HD7
Header : DNA REPAIR
Title : A Second Divalent Metal Ion in the Active Site of a New Crystal Form of Human Apurinic/Apyridinimic Endonuclease, Ape1, and its Implications for the Catalytic Mechanism
Release Data : 2001-02-16
Compound :
mol_id molecule chains synonym
1 DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE A AP ENDONUCLEASE 1, HAP1, REF1, APE1
ec: 4.2.99.18
Source :
mol_id organism_scientific organism_common expression_system
1 HOMO SAPIENS  (taxid:9606) HUMAN ESCHERICHIA COLI  (taxid:469008)
tissue: ALL
cell: ALL
organelle: NUCLEUS
expression_system_strain: BL21(DE3)
expression_system_plasmid: PET11D
expression_system_gene: APE1
Authors : Beernink, P.T., Segelke, B.W., Rupp, B.
Keywords : DNA REPAIR, ENDONUCLEASE, APE1, HAP1, REF-1
Exp. method : X-RAY DIFFRACTION ( 1.95 Å )
Citation :

Two Divalent Metal Ions in the Active Site of a New Crystal Form of Human Apurinic/Apyrimidinic Endonuclease, Ape1: Implications for the Catalytic Mechanism

Beernink, P.T.,Segelke, B.W.,Hadi, M.Z.  et al.
(2001)  J.Mol.Biol.  307 : 1023

PubMed: 11286553
DOI: 10.1006/JMBI.2001.4529

Chain : A
UniProt : P27695 (APEX1_HUMAN)
Reaction : The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.