Brand  (β version)

color scheme of protein:



Code Name Link
LI Lithium ion
CIT Citric acid
Code : 1H4D
Header : molybdenum cofactor biosynthesis
Title : biochemical and structural analysis of the molybdenum cofactor biosynthesis protein moba
Release Data : 2003-05-08
Compound :
mol_id 1
molecule molybdopterin-guanine dinucleotide biosynthesis protein a
chain A
synonym protein fa
engineered yes
mutation yes
other_details the full length construct was c-terminally extended with a 7-residue nickel affinity tag of sequence ser-his-his-his-his-his-his.
Source :
mol_id 1
organism_scientific Escherichia coli
organism_taxid 562
strain jm109
variant m15[prep4]
expression_system Escherichia coli
expression_system_taxid 562
expression_system_strain jm109
expression_system_variant m15[prep4]
expression_system_plasmid pkk223-3
other_details mutation introduced by 3-way pcr andverified by dna sequencing
Authors : A.Guse, C.E.M.Stevenson, J.Kuper, G.Buchanan, G.Schwarz, R.R.Mendel, D.M.Lawson, T.Palmer
Keywords : molybdopterin nucleotidyl-transferase, molybdenum cofactor biosynthesis, gtp-binding
Exp. method : X-ray diffraction ( 1.74 Å )
Chain : A
UniProt : P32173 (MOBA_ECOLI)
Reaction : GTP + molybdenum cofactor = diphosphate + guanylyl molybdenum cofactor.