Het-PDB Navi2 (PDB: 1H2U)

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Ligands
Code	Name		Style	Show	Link
GDP	Guanosine-5'-diphosphate				PoSSuM
7MG	7n-methyl-8-hydroguanosine-5'-monophosphate				PoSSuM

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Download interaction data: 1H2U

Code :

1H2U PDBj RCSB PDB PDBe

Header :

NUCLEAR PROTEIN

Title :

Structure of the human nuclear cap-binding-complex (CBC) in complex with a cap analogue m7GpppG

Release Data :

2002-10-17

Compound :

mol_id	molecule	chains	synonym
1	80 KDA NUCLEAR CAP BINDING PROTEIN	A,B	NCBP 80 KDA SUBUNIT, CBP80
	fragment: MIF4G DOMAIN, RESIDUES 20-652,701-790 mutation: YES other_details: DELETION OF THE FIRST 19 RESIDUES IN N-TERMINAL AND DELETION OF RESIDUES 653-701 REPLACED BY A GLYCINE, ENGINEERED MUTATION ALA 479 SER

mol_id	molecule	chains	synonym
2	20 KDA NUCLEAR CAP BINDING PROTEIN	X,Y	CBP20, NCBP 20 KDA SUBUNIT, NCBP INTERACTING PROTEIN 1, NIP1

Source :

mol_id	organism_scientific	organism_common	expression_system
1	HOMO SAPIENS (taxid:9606)	HUMAN	TRICHOPLUSIA NI (taxid:7111)
	expression_system_cell_line: High Five expression_system_vector_type: BACULOVIRUS

mol_id	organism_scientific	organism_common	expression_system
2	HOMO SAPIENS (taxid:9606)	HUMAN	ESCHERICHIA COLI BL21 (taxid:511693)
	expression_system_plasmid: PRSETA

Authors :

Mazza, C., Segref, A., Mattaj, I.W., Cusack, S.

Keywords :

M7GCAP, CAP-BINDING-COMPLEX, RNP DOMAIN, MIF4G DOMAIN, RNA MATURATION, RNA EXPORT, NUCLEAR PROTEIN, RNA-BINDING

Exp. method :

X-RAY DIFFRACTION ( 2.40 Å )

Citation :

Large-Scale Induced Fit Recognition of an M(7)Gpppg CAP Analogue by the Human Nuclear CAP-Binding Complex

Mazza, C.,Segref, A.,Mattaj, I.W. et al.
(2002) Embo J. 21 : 5548

Co-Crystallization of the Human Nuclear CAP-Binding Complex with a M7Gpppg CAP Analogue Using Protein Engineering

Mazza, C.,Segref, A.,Mattaj, I. et al.
(2002) Acta Crystallogr.,Sect.D 58 : 2194

PDB ID: 1H2U