Brand  (β version)

PDB ID: 1H2M

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Ligands
Code Name Style Show Link
OGA N-oxalylglycine PoSSuM
SO4 Sulfate ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Glycosylation
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Modification
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Structure summary

Code : 1H2M   PDBj   RCSB PDB   PDBe
Header : TRANSCRIPTION ACTIVATOR/INHIBITOR
Title : Factor Inhibiting HIF-1 alpha in complex with HIF-1 alpha fragment peptide
Release Data : 2002-11-28
Compound :
mol_id molecule chains synonym
1 FACTOR INHIBITING HIF1 A FIH1
mol_id molecule chains synonym
2 HYPOXIA-INDUCIBLE FACTOR 1 ALPHA S HIF-1 ALPHA, ARNT INTERACTING PROTEIN, MEMBER OF PAS PROTEIN 1, MOP1, HIF1 ALPHA, HIF1A.
fragment: C-TERMINAL TRANSACTIVATION DOMAIN FRAGMENT, RESIDUES 775-826
Source :
mol_id organism_scientific organism_common expression_system
1 HOMO SAPIENS  (taxid:9606) HUMAN ESCHERICHIA COLI  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_plasmid: PET28A(+)
mol_id organism_scientific organism_common expression_system
2 HOMO SAPIENS  (taxid:9606) HUMAN ESCHERICHIA COLI  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_plasmid: PGEX-GP-1
Authors : Elkins, J.M., Hewitson, K.S., McNeill, L.A., Schlemminger, I., Seibel, J.F., Schofield, C.J.
Keywords : TRANSCRIPTION ACTIVATOR-INHIBITOR COMPLEX, FIH, HIF, DSBH, OXYGENASE, TRANSCRIPTION, HYPOXIA, 2- OXOGLUTARATE, ASPARAGINYL HYDROXYLASE, HYDROXYLASE
Exp. method : X-RAY DIFFRACTION ( 2.50 Å )
Citation :

Structure of Factor-Inhibiting Hypoxia-Inducible Factor (Hif) Reveals Mechanism of Oxidative Modification of Hif-1Alpha

Elkins, J.M.,Hewitson, K.S.,McNeill, L.A.  et al.
(2003)  J.Biol.Chem.  278 : 1802 - 1806

PubMed: 12446723
DOI: 10.1074/JBC.C200644200

Reaction

Chain : A
UniProt : Q969Q7 (HIF1N_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
2-oxoglutarate + L-asparaginyl-[hypoxia-inducible factor alpha subunit] + O2 = (3S)-3-hydroxy-L-asparaginyl-[hypoxia-inducible factor alpha subunit] + CO2 + succinate 1.14.11.30 PubMed:12042299, PubMed:12080085, PubMed:14701857, PubMed:17573339
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2-oxoglutarate + L-histidyl-[ankyrin-repeat domain protein] + O2 = (3S)-3-hydroxy-L-histidyl-[ankyrin-repeat domain protein] + CO2 + succinate 1.14.11.n4 PubMed:21251231
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2-oxoglutarate + L-asparaginyl-[ankyrin-repeat domain protein] + O2 = (3S)-3-hydroxy-L-asparaginyl-[ankyrin-repeat domain protein] + CO2 + succinate 1.14.11.n4 PubMed:17003112, PubMed:17573339, PubMed:18299578, PubMed:19245366, PubMed:21177872
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2-oxoglutarate + L-aspartyl-[ankyrin-repeat domain protein] + O2 = (3S)-3-hydroxy-L-aspartyl-[ankyrin-repeat domain protein] + CO2 + succinate 1.14.11.n4 PubMed:21177872
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Chain : S
UniProt : Q16665 (HIF1A_HUMAN)
Reaction : -