Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
DA 2'-deoxyadenosine-5'-monophosphate
Code : 1GIS
Header : HYDROLASE
Title : A TRICHOSANTHIN(TCS) MUTANT(E85Q) COMPLEX STRUCTURE WITH 2'-DEOXY-ADENOSIN-5'-MONOPHOSPHATE
Release Data : 2003-06-03
Compound :
mol_id molecule chains
1 RIBOSOME-INACTIVATING PROTEIN ALPHA-TRICHOSANTHIN A
ec: 3.2.2.22
mutation: E86Q
Source :
mol_id organism_scientific expression_system
1 Trichosanthes kirilowii  (taxid:3677) Escherichia coli  (taxid:562)
Authors : Guo, Q., Liu, Y., Dong, Y., Rao, Z.
Keywords : PROTEIN-SUB COMPLEX, TRICHOSANTHIN, TCS, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.7 Å )
Citation :

Substrate binding and catalysis in trichosanthin occur in different sites as revealed by the complex structures of several E85 mutants.

Guo, Q.,Zhou, W.,Too, H.M.  et al.
(2003)  Protein Eng.  16 : 391 - 396

PubMed: 12874371
DOI: 10.1093/protein/gzg056

Chain : A
UniProt : P09989 (RIPT_TRIKI)
Reaction : Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.