Het-PDB Navi2 (PDB: 1GEB)

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Ligands
Code	Name		Style	Show	Link
CAM	Camphor				PoSSuM
HEM	Protoporphyrin ix containing Fe				PoSSuM

Non-standard Residues
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Glycosylation
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Download interaction data: 1GEB

Code :

1GEB PDBj RCSB PDB PDBe

Header :

ELECTRON TRANSPORT

Title :

X-RAY CRYSTAL STRUCTURE AND CATALYTIC PROPERTIES OF THR252ILE MUTANT OF CYTOCHROME P450CAM

Release Data :

2000-11-15

Compound :

mol_id	molecule	chains
1	CYTOCHROME P450-CAM	A
	ec: 1.14.15.1 mutation: T252I

Source :

mol_id	organism_scientific	expression_system
1	Pseudomonas putida (taxid:303)	Escherichia coli (taxid:562)
	expression_system_strain: JM109 expression_system_vector_type: PLASMID expression_system_plasmid: PUC19

Authors :

Hishiki, T., Shimada, H., Nagano, S., Park, S.-Y., Ishimura, Y.

Keywords :

Cytochrome P450cam, Monooxygenase, ELECTRON TRANSPORT

Exp. method :

X-RAY DIFFRACTION ( 2.03 Å )

Citation :

X-ray crystal structure and catalytic properties of Thr252Ile mutant of cytochrome P450cam: roles of Thr252 and water in the active center.

Hishiki, T.,Shimada, H.,Nagano, S. et al.
(2000) J.Biochem. 128 : 965 - 974

Chain :

UniProt :

P00183 (CPXA_PSEPU)

Reaction:	EC:	Evidence:
Reaction:	Physiological Direction:	Evidence:
(1R,4R)-camphor + 2 H(+) + O2 + 2 reduced [2Fe-2S]- [putidaredoxin] = (1R,4R,5R)-5-hydroxycamphor + H2O + 2 oxidized [2Fe-2S]-[putidaredoxin]	1.14.15.1	-
	-	-

PDB ID: 1GEB