Brand  (β version)

PDB ID: 1G8M

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Ligands
Code Name Style Show Link
5GP Guanosine-5'-monophosphate PoSSuM
K Potassium ion PoSSuM
Non-standard Residues
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MSE Selenomethionine
Glycosylation
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Structure summary

Code : 1G8M   PDBj   RCSB PDB   PDBe
Header : transferase, hydrolase
Title : CRYSTAL STRUCTURE OF AVIAN ATIC, A BIFUNCTIONAL TRANSFORMYLASE AND CYCLOHYDROLASE ENZYME IN PURINE BIOSYNTHESIS AT 1.75 ANG. RESOLUTION
Release Data : 2001-04-27
Compound :
mol_id molecule chains synonym
1 AICAR TRANSFORMYLASE-IMP CYCLOHYDROLASE A,B BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH [INCLUDES PHOSPHORIBOSYLAMINOIMIDAZOLECARBOXAMIDE FORMYLTRANSFERASE (AICAR TRANSFORMYLASE) AND IMP CYCLOHYDROLASE (INOSINICASE, IMP SYNTHETASE, ATIC)]
ec: 2.1.2.3, 3.5.4.10
fragment: AMINOIMIDAZOLE CARBOXAMIDE RIBONUCLEOTIDE TRANSFORMYLASE - INOSINE MONOPHOSPHATE CYCLOHYDROLASE
mutation: MET REPLACED BY SE-MET (MSE)
Source :
mol_id organism_scientific organism_common expression_system
1 Gallus gallus  (taxid:9031) Chicken Escherichia coli  (taxid:562)
gene: PURH
expression_system_strain: B834(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET 28A
Authors : Greasley, S.E., Horton, P., Beardsley, G.P., Benkovic, S.J., Wilson, I.A.
Keywords : Homodimer, 2 functional domains; IMPCH domain = alpha/beta/alpha; AICAR Tfase = 2 alpha/beta/alpha domains, 1 alpha + beta domain, transferase, hydrolase
Exp. method : X-RAY DIFFRACTION ( 1.75 Å )
Citation :

Crystal structure of a bifunctional transformylase and cyclohydrolase enzyme in purine biosynthesis.

Greasley, S.E.,Horton, P.,Ramcharan, J.  et al.
(2001)  Nat.Struct.Biol.  8 : 402 - 406

PubMed: 11323713
DOI: 10.1038/87555

Reaction

Chain : A, B
UniProt : P31335 (PUR9_CHICK)
Reaction: EC: Evidence:
Physiological Direction:
(6R)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D- ribosyl)imidazole-4-carboxamide = 5-formamido-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide + (6S)-5,6,7,8-tetrahydrofolate 2.1.2.3 PubMed:12501179
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10-formyldihydrofolate + 5-amino-1-(5-phospho-beta-D- ribosyl)imidazole-4-carboxamide = 5-formamido-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide + 7,8-dihydrofolate - UniProtKB:P31939
left-to-right
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4- carboxamide 3.5.4.10 UniProtKB:P31939
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