Brand  (β version)

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Modified Residues
Code Name Link
MSE Selenomethionine
N Any 5'-monophosphate nucleotide
Code : 1FYK
Header : TRANSCRIPTION/DNA
Title : SERENDIPITOUS CRYSTAL STRUCTURE CONTAINING THE HEAT SHOCK TRANSCRIPTION FACTOR'S DNA BINDING DOMAIN AND COGNATE DNA THAT IS TRANSLATIONALLY DISORDERED
Release Data : 2001-09-28
Compound :
mol_id molecule chains
1 HSE DNA-PHOSPHATE BACKBONE C
mol_id molecule chains synonym
2 HEAT SHOCK FACTOR PROTEIN A HEAT SHOCK TRANSCRIPTION FACTOR
fragment: DNA BINDING DOMAIN
mutation: N282R, F283H, K284A
Source :
mol_id organism_scientific
1
synthetic: yes
other_details: This sequence is based on an idealized HSE sequence.
mol_id organism_scientific expression_system
2 Kluyveromyces lactis  (taxid:28985) Escherichia coli  (taxid:562)
expression_system_vector_type: PLASMID
expression_system_plasmid: PHN280R
Authors : Littlefield, O., Nelson, H.C.M.
Keywords : crystal-packing interface, crystallization, protein-DNA interface, protein-protein interface, static disorder, TRANSCRIPTION-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.5 Å )
Citation :

Crystal packing interaction that blocks crystallization of a site-specific DNA binding protein-DNA complex.

Littlefield, O.,Nelson, H.C.
(2001)  Proteins  45 : 219 - 228

PubMed: 11599025
DOI: 10.1002/prot.1142

A new use for the 'wing' of the 'winged' helix-turn-helix motif in the HSF-DNA cocrystal

Littlefield, O.,Nelson, H.C.M.
(1999)  Nat.Struct.Biol.  6 : 464 - 470

DOI: 10.1038/8269

Chain : A
UniProt : P22121 (HSF_KLULA)
Reaction : -