Brand  (β version)

PDB ID: 1FUY

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Ligands
Code Name Style Show Link
FIP 5-fluoroindole propanol phosphate PoSSuM
NA Sodium ion PoSSuM
PLP Pyridoxal-5'-phosphate PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1FUY   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : CRYSTAL STRUCTURE OF BETAA169L/BETAC170W DOUBLE MUTANT OF TRYPTOPHAN SYNTHASE COMPLEXED WITH 5-FLUORO-INDOLE-PROPANOL PHOSPHATE
Release Data : 2001-01-17
Compound :
mol_id molecule chains
1 TRYPTOPHAN SYNTHASE ALPHA CHAIN A
ec: 4.2.1.20
mol_id molecule chains
2 TRYPTOPHAN SYNTHASE BETA CHAIN B
ec: 4.2.1.20
mutation: R34S, A169L, C170W
Source :
mol_id organism_scientific expression_system
1 Salmonella typhimurium  (taxid:602) Escherichia coli  (taxid:562)
cell_line: CB149
gene: TRPA/TRPB
expression_system_vector_type: PLASMID
expression_system_plasmid: PSTB7
mol_id organism_scientific expression_system
2 Salmonella typhimurium  (taxid:602) Escherichia coli  (taxid:562)
cell_line: CB149
gene: TRPA/TRPB
expression_system_vector_type: PLASMID
expression_system_plasmid: PSTB7
Authors : Weyand, M., Schlichting, I.
Keywords : LYASE, CARBON-OXYGEN LYASE, TRYPTOPHAN BIOSYNTHESIS, PYRIDOXAL PHOSPHATE
Exp. method : X-RAY DIFFRACTION ( 2.25 Å )
Citation :

Structural basis for the impaired channeling and allosteric inter-subunit communication in the beta A169L/beta C170W mutant of tryptophan synthase.

Weyand, M.,Schlichting, I.
(2000)  J.Biol.Chem.  275 : 41058 - 41063

PubMed: 11034989
DOI: 10.1074/jbc.C000479200

Reaction

Chain : A
UniProt : P00929 (TRPA_SALTY)
Reaction: EC: Evidence:
Physiological Direction:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + L-tryptophan + H2O 4.2.1.20 HAMAP-Rule:MF_00131
-
Chain : B
UniProt : P0A2K1 (TRPB_SALTY)
Reaction: EC: Evidence:
Physiological Direction:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + L-tryptophan + H2O 4.2.1.20 -
-
Cofactor: Evidence: Note:
pyridoxal 5'-phosphate - -