Brand  (β version)

PDB ID: 1EQB

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
GLY Glycine PoSSuM
PLP Pyridoxal-5'-phosphate PoSSuM
FFO N-[4-({[(6s)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1EQB   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : X-RAY CRYSTAL STRUCTURE AT 2.7 ANGSTROMS RESOLUTION OF TERNARY COMPLEX BETWEEN THE Y65F MUTANT OF E-COLI SERINE HYDROXYMETHYLTRANSFERASE, GLYCINE AND 5-FORMYL TETRAHYDROFOLATE
Release Data : 2000-04-19
Compound :
mol_id molecule chains
1 SERINE HYDROXYMETHYLTRANSFERASE A,B,C,D
ec: 2.1.2.1
mutation: Y65F
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
expression_system_strain: GS245
expression_system_vector_type: PLASMID
expression_system_plasmid: PBR322
Authors : Contestabile, R., Angelaccio, S., Bossa, F., Wright, H.T., Scarsdale, N., Kazanina, G., Schirch, V.
Keywords : Functional mutant, hydroxymethyltransferase, aat-like fold, one carbon metabolism, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.70 Å )
Citation :

Role of tyrosine 65 in the mechanism of serine hydroxymethyltransferase.

Contestabile, R.,Angelaccio, S.,Bossa, F.  et al.
(2000)  Biochemistry  39 : 7492 - 7500

PubMed: 10858298
DOI: 10.1021/bi000032z

Crystal Structure at 2.4 Angstrom Resolution of E. Coli Serine Hydroxymethyltransferase in Complex with Glycine Substrate and 5-Formyl Tetrahydrofolate

Scarsdale, J.N.,Radaev, S.,Kazanina, G.  et al.
(2000)  J.Mol.Biol.  296 : 155 - 168

DOI: 10.1006/jmbi.1999.3453

Reaction

Chain : A, B, C, D
UniProt : P00477 (GLYA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine 2.1.2.1 HAMAP-Rule:MF_00051, PubMed:10858298, PubMed:12773539, PubMed:1517215, PubMed:19883126, PubMed:3891721, PubMed:7925461
-
L-allo-threonine = acetaldehyde + glycine - PubMed:10858298, PubMed:1517215, PubMed:19883126, PubMed:3891721
-
(6R)-5,10-methenyltetrahydrofolate + H2O = (6S)-5-formyl- 5,6,7,8-tetrahydrofolate + H(+) - PubMed:10858298, PubMed:2201683
-