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Ligands
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Non-standard Residues
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CRO {2-[(1r,2r)-1-amino-2-hydroxypropyl]-4-(4-hydroxybenzylidene)-5-oxo-4,5-dihydro-1h-imidazol-1-yl}acetic acid
Glycosylation
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Modification
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Code : 1EMG   PDBj   RCSB PDB   PDBe
Header : GREENFLUORESCENT PROTEIN
Title : GREEN FLUORESCENT PROTEIN (65-67 REPLACED BY CRO, S65T SUBSTITUTION, Q80R)
Release Data : 1999-05-12
Compound :
mol_id molecule chains
1 PROTEIN (GREEN FLUORESCENT PROTEIN) A
mutation: 65 - 67 REPLACED BY CRO, S65T SUBSTITUTION, Q80R SUBSTITUTION
Source :
mol_id organism_scientific expression_system
1 Aequorea victoria  (taxid:6100) Escherichia coli  (taxid:562)
strain: JM109(DE3)
organ: PHOTOGENIC ORGAN
tissue: CIRCUMORAL RING CANAL
expression_system_strain: JM109(DE3)
expression_system_cellular_location: CYTAPLASM
expression_system_vector_type: PLASMID
other_details: THE N-TERMINAL HIS-TAG HAS BEEN REMOVED
Authors : Elsliger, M.A., Wachter, R.M., Kallio, K., Hanson, G.T., Remington, S.J.
Keywords : GREENFLUORESCENT PROTEIN, BIOLUMINESCENCE, PHOTOACTIVE PROTEIN, FLUORESCENT TAG, PH TITRATION
Exp. method : X-RAY DIFFRACTION ( 2.0 Å )
Citation :

Structural and spectral response of green fluorescent protein variants to changes in pH.

Elsliger, M.A.,Wachter, R.M.,Hanson, G.T.  et al.
(1999)  Biochemistry  38 : 5296 - 5301

PubMed: 10220315
DOI: 10.1021/bi9902182

Spectral and Structural Response of Gfp Mutants to Variations in Ph and Ionic Strength

Wachter, R.M.,Elsliger-A, M.,Kallio, K.  et al.
To be Published 

Structural Basis of Spectral Shifts in the Yellow-Emission Variants of Green Fluorescent Protein

Wachter, R.M.,Elsliger-A, M.,Kallio, K.  et al.
(1998)  Structure  6 : 1267

Chain : A
UniProt : P42212 (GFP_AEQVI)
Reaction : -