Brand  (β version)

PDB ID: 1ECB

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Ligands
Code Name Style Show Link
MG Magnesium ion PoSSuM
5GP Guanosine-5'-monophosphate PoSSuM
Non-standard Residues
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Structure summary

Code : 1ECB   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 GMP, 1 MG PER SUBUNIT
Release Data : 1998-04-15
Compound :
mol_id molecule chains
1 GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE AMIDOTRANSFERASE A,B,C,D
ec: 2.4.2.14
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli BL21(DE3)  (taxid:469008)
gene: PURF
expression_system_strain: BL21 (DE3)
expression_system_plasmid: PT7F1
Authors : Krahn, J.M., Smith, J.L.
Keywords : TRANSFERASE, GLUTAMINE AMIDOTRANSFERASE, PURINE BIOSYNTHESIS, GLYCOSYLTRANSFERASE, GMP, GUANINE 5'-MONOPHOSPHATE
Exp. method : X-RAY DIFFRACTION ( 2.7 Å )
Citation :

Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site.

Krahn, J.M.,Kim, J.H.,Burns, M.R.  et al.
(1997)  Biochemistry  36 : 11061 - 11068

PubMed: 9333323
DOI: 10.1021/bi9714114

Reaction

Chain : A, B, C, D
UniProt : P00496 (PUR1_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5- phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine 2.4.2.14 HAMAP- Rule:MF_01931, PubMed:372191
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