Brand  (β version)

PDB ID: 1EA0

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Ligands
Code Name Style Show Link
OMT S-dioxymethionine PoSSuM
FMN Flavin mononucleotide PoSSuM
AKG 2-oxoglutaric acid PoSSuM
F3S Fe3-s4 cluster PoSSuM
Non-standard Residues
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Structure summary

Code : 1EA0   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Alpha subunit of A. brasilense glutamate synthase
Release Data : 2001-11-01
Compound :
mol_id molecule chains synonym
1 GLUTAMATE SYNTHASE [NADPH] LARGE CHAIN A,B GLUTAMATE SYNTHASE ALPHA SUBUNIT, NADPH-GOGAT, GLTS ALPHA CHAIN
ec: 1.4.1.13
Source :
mol_id organism_scientific expression_system
1 AZOSPIRILLUM BRASILENSE  (taxid:192) ESCHERICHIA COLI  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_plasmid: PET11A
Authors : Binda, C., Bossi, R.T., Vanoni, M.A., Mattevi, A.
Keywords : OXIDOREDUCTASE, IRON SULPHUR FLAVOPROTEIN
Exp. method : X-RAY DIFFRACTION ( 3.0 Å )
Citation :

Cross-Talk and Ammonia Channeling between Active Centers in the Unexpected Domain Arrangement of Glutamate Synthase

Binda, C.,Bossi, R.T.,Wakatsuki, S.  et al.
(2000)  Structure  8 : 1299

PubMed: 11188694
DOI: 10.1016/S0969-2126(00)00540-2

Reaction

Chain : A, B
UniProt : Q05755 (GLTB_AZOBR)
Reaction: EC: Evidence:
Physiological Direction:
2 L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + L-glutamine + NADPH 1.4.1.13 -
-