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Ligands
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CIT Citric acid
LI Lithium ion
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Code : 1E5K   PDBj   RCSB PDB   PDBe
Header : MOLYBDOPTERIN NUCLEOTIDYL-TRANSFERASE
Title : CRYSTAL STRUCTURE OF THE MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN MOBA (PROTEIN FA) FROM ESCHERICHIA COLI AT NEAR ATOMIC RESOLUTION
Release Data : 2000-11-07
Compound :
mol_id molecule chains synonym
1 MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS PROTEIN A A MOBA, PROTEIN FA
other_details: THE WILD-TYPE CONSTRUCT WAS C-TERMINALLY EXTENDED WITH A 7-RESIDUE NICKEL AFFINITY TAG OF SEQUENCE SER-HIS-HIS-HIS-HIS-HIS-HIS.
Source :
mol_id organism_scientific expression_system
1 ESCHERICHIA COLI  (taxid:83333) ESCHERICHIA COLI  (taxid:562)
strain: K12
cellular_location: CYTOPLASM
gene: MOBA
expression_system_strain: JM109
expression_system_variant: M15[PREP4]
expression_system_cellular_location: CYTOPLASM
expression_system_vector_type: PLASMID
expression_system_plasmid: PKK223-3
expression_system_gene: MOBA
Authors : Stevenson, C.E.M., Sargent, F., Buchanan, G., Palmer, T., Lawson, D.M.
Keywords : MOLYBDOPTERIN NUCLEOTIDYL-TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.35 Å )
Citation :

Crystal Structure of the Molybdenum Cofactor Biosynthesis Protein Moba from Escherichia Coli at Near Atomic Resolution

Stevenson, C.E.M.,Sargent, F.,Buchanan, G.  et al.
(2000)  Structure  8 : 1115

PubMed: 11080634
DOI: 10.1016/S0969-2126(00)00518-9

Chain : A
UniProt : P32173 (MOBA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
GTP + H(+) + Mo-molybdopterin = diphosphate + Mo-molybdopterin guanine dinucleotide 2.7.7.77 PubMed:10978348, PubMed:21081498
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