Brand  (β version)

PDB ID: 1E14

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Ligands
Code Name Style Show Link
BCL Bacteriochlorophyll a PoSSuM
BPH Bacteriopheophytin a PoSSuM
CDL Cardiolipin PoSSuM
FE Fe (III) ion PoSSuM
LDA Lauryl dimethylamine-N-oxide PoSSuM
SPN Speroidenone PoSSuM
U10 Ubiquinone-10 PoSSuM
Non-standard Residues
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Glycosylation
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Modification
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Structure summary

Code : 1E14   PDBj   RCSB PDB   PDBe
Header : ELECTRON TRANSPORT
Title : PHOTOSYNTHETIC REACTION CENTER MUTANT WITH PHE M197 REPLACED WITH ARG (CHAIN M, FM197R) AND GLY M203 REPLACED WITH ASP (CHAIN M, GM203D)
Release Data : 2000-06-02
Compound :
mol_id molecule chains synonym
1 Reaction center protein H chain H Photosynthetic reaction center H subunit
mol_id molecule chains synonym
2 Reaction center protein L chain L Photosynthetic reaction center L subunit
mol_id molecule chains synonym
3 Reaction center protein M chain M Photosynthetic reaction center M subunit
mutation: YES
Source :
mol_id organism_scientific expression_system
1 Rhodobacter sphaeroides  (taxid:1063) RHODOBACTER SPHAEROIDES  (taxid:1063)
atcc: NCIB 8253
cellular_location: CYTOPLASMIC MEMBRANE
gene: puhA
expression_system_strain: WM115F/FM197R
expression_system_vector_type: PLASMID
expression_system_plasmid: PRKEH10D
expression_system_gene: PUFQLMX
mol_id organism_scientific expression_system
2 Rhodobacter sphaeroides  (taxid:1063) RHODOBACTER SPHAEROIDES  (taxid:1063)
atcc: NCIB 8253
cellular_location: CYTOPLASMIC MEMBRANE
gene: pufL
expression_system_strain: WM115F/FM197R
expression_system_vector_type: PLASMID
expression_system_plasmid: PRKEH10D
expression_system_gene: PUFQLMX
mol_id organism_scientific expression_system
3 Rhodobacter sphaeroides  (taxid:1063) RHODOBACTER SPHAEROIDES  (taxid:1063)
atcc: NCIB 8253
cellular_location: CYTOPLASMIC MEMBRANE
gene: pufM
expression_system_strain: WM115F/FM197R
expression_system_vector_type: PLASMID
expression_system_plasmid: PRKEH10D
expression_system_gene: PUFQLMX
Authors : Fyfe, P.K., Ridge, J.P., McAuley, K.E., Cogdell, R.J., Isaacs, N.W., Jones, M.R.
Keywords : TRANSMEMBRANE, ELECTRON TRANSPORT, PHOTOSYNTHESIS
Exp. method : X-RAY DIFFRACTION ( 2.7 Å )
Citation :

Structural Consequences of the Replacement of Glycine M203 with Aspartic Acid in the Reaction Center from Rhodobacter Sphaeroides.

Fyfe, P.K.,Ridge, J.P.,Mcauley, K.E.  et al.
(2000)  Biochemistry  39 : 5953

PubMed: 10821666
DOI: 10.1021/BI9925017

Structural Details of an Interaction between Cardiolipin and an Integral Membrane Protein

Mcauley, K.E.,Fyfe, P.K.,Ridge, J.P.  et al.
(1999)  Proc.Natl.Acad.Sci.USA  96 : 14706

PubMed: 10611277
DOI: 10.1073/PNAS.96.26.14706

Structural Studies of Wild-Type and Mutant Reaction Centers from an Antenna-Deficient Strain of Rhodobacter Sphaeroides: Monitoring the Optical Properties of the Complex from Bacterial Cell to Crystal

Mcauley, K.E.,Fyfe, P.K.,Ridge, J.P.  et al.
(1998)  Biochemistry  37 : 4740

PubMed: 9537989
DOI: 10.1021/BI971717A

Reaction

Chain : H
UniProt : P0C0Y7 (RCEH_CERSP)
Reaction : -
Cofactor: Evidence: Note:
a bacteriochlorophyll - Binds 4 bacteriochlorophylls per trimer.
a bacteriopheophytin - Binds 2 bacteriopheophytins per trimer.
Fe cation - Binds 1 Fe cation per trimer.
Mg(2+) - Binds 4 Mg(2+) ions per trimer.
a ubiquinone - Binds 2 ubiquinone per trimer.
Chain : L
UniProt : P0C0Y8 (RCEL_CERSP)
Reaction : -
Chain : M
UniProt : P0C0Y9 (RCEM_CERSP)
Reaction : -