Brand  (β version)

PDB ID: 1DWX

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
HEM Protoporphyrin ix containing Fe PoSSuM
H4B 5,6,7,8-tetrahydrobiopterin PoSSuM
SO4 Sulfate ion PoSSuM
HAR N-omega-hydroxy-L-arginine PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1DWX   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE/TRANSFERASE
Title : MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER N-hydroxyarginine and tetrahydrobiopterin
Release Data : 2000-02-06
Compound :
mol_id molecule chains synonym
1 NITRIC OXIDE SYNTHASE A,B INDUCIBLE NOS TYPE II, MAC-NOS
ec: 1.14.13.39
fragment: OXYGENASE DOMAIN 65-498
other_details: MURINE INDUCIBLE
Source :
mol_id organism_scientific organism_common expression_system
1 MUS MUSCULUS  (taxid:10090) MOUSE ESCHERICHIA COLI  (taxid:562)
cell: MACROPHAGE
expression_system_plasmid: PCWORI
Authors : Crane, B.R., Arvai, A.S., Getzoff, E.D., Stuehr, D.J., Tainer, J.A.
Keywords : OXIDOREDUCTASE TRANSFERASE COMPLEX, COMPLEX (OXIDOREDUCTASE-TRANSFERASE), NITRIC OXIDE MONOOXYGENASE, HEME, DIMER, INTERMEDIATE, PTERIN, H4B, TETRAHYDROBIOPTERIN, OXIDOREDUCTASE-TRANSFERASE complex
Exp. method : X-RAY DIFFRACTION ( 2.60 Å )
Citation :

Structures of the N(Omega)-Hydroxy-L-Arginine Complex of Inducible Nitric Oxide Synthase Oxygenase Dimer with Active Andinactive Pterins

Crane, B.R.,Arvai, A.S.,Ghosh, S.  et al.
(2000)  Biochemistry  39 : 4608

PubMed: 10769116
DOI: 10.1021/BI992409A

N-Terminal Domain Swapping and Metal Ion Binding in Nitric Oxide Synthase Dimerization

Crane, B.R.,Rosenfeld, R.A.,Arvai, A.S.  et al.
(1999)  Embo J.  18 : 6271

PubMed: 10562539
DOI: 10.1093/EMBOJ/18.22.6271

Inducible Nitric Oxide Synthase: Role of the N-Terminal Beta-Hairpin Hook and Pterin-Binding Segment in Dimerization and Tetrahydrobiopterin Interaction

Ghosh, D.K.,Crane, B.R.,Ghosh, S.  et al.
(1999)  Embo J.  18 : 6260

PubMed: 10562538
DOI: 10.1093/EMBOJ/18.22.6260

Structure of Nitric Oxide Synthase Oxygenase Dimer with Pterin and Substrate

Crane, B.R.,Arvai, A.S.,Ghosh, D.K.  et al.
(1998)  Science  279 : 2121

PubMed: 9516116
DOI: 10.1126/SCIENCE.279.5359.2121

The Structure of Nitric Oxide Synthase Oxygenase Domain and Inhibitor Complexes

Crane, B.R.,Arvai, A.S.,Gachhui, R.  et al.
(1997)  Science  278 : 425

PubMed: 9334294
DOI: 10.1126/SCIENCE.278.5337.425

Reaction

Chain : A, B
UniProt : P29477 (NOS2_MOUSE)
Reaction: EC: Evidence:
Physiological Direction:
H(+) + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline + 3 NADP(+) + 2 nitric oxide 1.14.13.39 UniProtKB:P35228
left-to-right