Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
SR Strontium ion
DUD Deoxyuridine-5'-diphosphate
Code : 1DUC
Header : HYDROLASE
Title : EIAV DUTPASE DUDP/STRONTIUM COMPLEX
Release Data : 1998-06-03
Compound :
mol_id molecule chains synonym
1 DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE A DUTPASE, DUTP PYROPHOSPHATASE
ec: 3.6.1.23
other_details: COMPLEX WITH DUDP AND STRONTIUM(II) ION
Source :
mol_id organism_scientific expression_system
1 Equine infectious anemia virus  (taxid:11665) Escherichia coli  (taxid:562)
cell_line: BL21
expression_system_strain: BL21 (DE3) PLYSS
expression_system_plasmid: PET-3A/EDU
Authors : Dauter, Z., Persson, R., Rosengren, A.M., Nyman, P.O., Wilson, K.S., Cedergren-Zeppezauer, E.S.
Keywords : HYDROLASE, DUTPASE, EIAV, TRIMERIC ENZYME, INHIBITOR COMPLEX, ASPARTYL PROTEASE
Exp. method : X-RAY DIFFRACTION ( 2.05 Å )
Citation :

Crystal structure of dUTPase from equine infectious anaemia virus; active site metal binding in a substrate analogue complex.

Dauter, Z.,Persson, R.,Rosengren, A.M.  et al.
(1999)  J.Mol.Biol.  285 : 655 - 673

PubMed: 9878436
DOI: 10.1006/jmbi.1998.2332

Chain : A
UniProt : P11204 (POL_EIAV9)
Reaction : Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).