Brand  (β version)

PDB ID: 1DFO

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Ligands
Code Name Style Show Link
PLG N-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane] PoSSuM
FFO N-[4-({[(6s)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid PoSSuM
Non-standard Residues
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Structure summary

Code : 1DFO   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : CRYSTAL STRUCTURE AT 2.4 ANGSTROM RESOLUTION OF E. COLI SERINE HYDROXYMETHYLTRANSFERASE IN COMPLEX WITH GLYCINE AND 5-FORMYL TETRAHYDROFOLATE
Release Data : 1999-12-10
Compound :
mol_id molecule chains synonym
1 SERINE HYDROXYMETHYLTRANSFERASE A,B,C,D SHMT, SERINE METHYLASE
ec: 2.1.2.1
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
expression_system_strain: GS1993 (GLYA-, PHEA905, DELTA LACU169,STRA,THI, DELTAGLYA::MU, RECA-)
expression_system_vector_type: PLASMID
expression_system_plasmid: PBR322
Authors : Scarsdale, J.N., Radaev, S., Kazanina, G., Schirch, V., Wright, H.T.
Keywords : ALPHA PLP ASPARTATE, AMINO TRANSFERASE, (AAT)-LIKE FOLD, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.40 Å )
Citation :

Crystal structure at 2.4 A resolution of E. coli serine hydroxymethyltransferase in complex with glycine substrate and 5-formyl tetrahydrofolate.

Scarsdale, J.N.,Radaev, S.,Kazanina, G.  et al.
(2000)  J.Mol.Biol.  296 : 155 - 168

PubMed: 10656824
DOI: 10.1006/jmbi.1999.3453

Reaction

Chain : A, B, C, D
UniProt : P0A825 (GLYA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine 2.1.2.1 HAMAP-Rule:MF_00051, PubMed:10858298, PubMed:12773539, PubMed:1517215, PubMed:19883126, PubMed:3891721, PubMed:7925461
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L-allo-threonine = acetaldehyde + glycine - PubMed:10858298, PubMed:1517215, PubMed:19883126, PubMed:3891721
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(6R)-5,10-methenyltetrahydrofolate + H2O = (6S)-5-formyl- 5,6,7,8-tetrahydrofolate + H(+) - PubMed:10858298, PubMed:2201683
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