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Ligands
Code Name Style Show Link
COA Coenzyme a
SO4 Sulfate ion
Non-standard Residues
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CSD 3-sulfinoalanine
Glycosylation
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Modification
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Code : 1D6H   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : CHALONE SYNTHASE (N336A MUTANT COMPLEXED WITH COA)
Release Data : 2000-02-03
Compound :
mol_id molecule chains
1 CHALCONE SYNTHASE A
ec: 2.3.1.74
mutation: N336A
Source :
mol_id organism_scientific expression_system
1 Medicago sativa  (taxid:3879) Escherichia coli  (taxid:562)
expression_system_vector: PHIS8 (VARIANT OF PET-28B)
Authors : Jez, J.M., Ferrer, J.L., Bowman, M.E., Dixon, R.A., Noel, J.P.
Keywords : POLYPETIDE SYNTHASE, FLAVONOID BIOSYNTHESIS, MALONYL-COA DECARBOXYLATION, SITE-DIRECTED MUTANT, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.15 Å )
Citation :

Dissection of malonyl-coenzyme A decarboxylation from polyketide formation in the reaction mechanism of a plant polyketide synthase.

Jez, J.M.,Ferrer, J.L.,Bowman, M.E.  et al.
(2000)  Biochemistry  39 : 890 - 902

PubMed: 10653632
DOI: 10.1021/bi991489f

Chain : A
UniProt : P30074 (CHS2_MEDSA)
Reaction: EC: Evidence:
Physiological Direction:
4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'- tetrahydroxychalcone + 3 CO2 + 4 CoA 2.3.1.74 PROSITE-ProRule:PRU10023
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