Brand  (β version)

PDB ID: 1CXL

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
MPD (4s)-2-methyl-2,4-pentanediol PoSSuM
G4D 4-deoxy-alpha-D-glucopyranose PoSSuM
GLC Alpha-D-glucopyranose PoSSuM
GLF Alpha-D-glucopyranosyl fluoride PoSSuM
: Polysaccharide
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1CXL   PDBj   RCSB PDB   PDBe
Header : GLYCOSYLTRANSFERASE
Title : COMPLEX BETWEEN A COVALENT INTERMEDIATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q
Release Data : 1999-05-03
Compound :
mol_id molecule chains synonym
1 PROTEIN (CYCLODEXTRIN-GLYCOSYLTRANSFERASE) A CGTASE
ec: 2.4.1.19
mutation: E257Q
other_details: COVALENTLY BOUND REACTION INTERMEDIATE
Source :
mol_id organism_scientific expression_system
1 Bacillus circulans  (taxid:1397) Bacillus subtilis  (taxid:1423)
strain: 251
expression_system_strain: DB104A
expression_system_plasmid: PDP66S
expression_system_gene: CGT
other_details: MUTANTS WERE CONSTRUCTED IN ECOLI STRAIN MC1061
Authors : Uitdehaag, J.C.M., Dijkstra, B.W.
Keywords : GLYCOSYL HYDROLASE FAMILY 13, ALPHA-AMYLASE FAMILY, INTERMEDIATE COMPLEX, 4- DEOXYMALTOTRIOSE, GLYCOSYLTRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.810 Å )
Citation :

X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family.

Uitdehaag, J.C.,Mosi, R.,Kalk, K.H.  et al.
(1999)  Nat.Struct.Biol.  6 : 432 - 436

PubMed: 10331869
DOI: 10.1038/8235

Reassessment of acarbose as a transition state analogue inhibitor of cyclodextrin glycosyltransferase.

Mosi, R.,Sham, H.,Uitdehaag, J.C.  et al.
(1998)  Biochemistry  37 : 17192 - 17198

PubMed: 9860832
DOI: 10.1021/bi981109a

Structure of cyclodextrin glycosyltransferase complexed with a maltononaose inhibitor at 2.6 angstrom resolution. Implications for product specificity.

Strokopytov, B.,Knegtel, R.M.,Penninga, D.  et al.
(1996)  Biochemistry  35 : 4241 - 4249

PubMed: 8672460
DOI: 10.1021/bi952339h

Crystallographic studies of the interaction of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 with natural substrates and products.

Knegtel, R.M.,Strokopytov, B.,Penninga, D.  et al.
(1995)  J.Biol.Chem.  270 : 29256 - 29264

PubMed: 7493956
DOI: 10.1074/jbc.270.49.29256

Nucleotide sequence and X-ray structure of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 in a maltose-dependent crystal form.

Lawson, C.L.,van Montfort, R.,Strokopytov, B.  et al.
(1994)  J.Mol.Biol.  236 : 590 - 600

PubMed: 8107143
DOI: 10.1006/jmbi.1994.1168

Reaction

Chain : A
UniProt : ?
Reaction : -