Brand  (β version)

PDB ID: 1CMJ

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
HEM Protoporphyrin ix containing Fe PoSSuM
NO Nitric oxide PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1CMJ   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : CRYSTAL STRUCTURES OF FERRIC-NO COMPLEXES OF FUNGAL NITRIC OXIDE REDUCTASE AND THEIR SER286 MUTANTS AT CRYOGENIC TEMPERATURE
Release Data : 2000-03-22
Compound :
mol_id molecule chains synonym
1 CYTOCHROME P450 A P450NOR
ec: 1.14.-.-
mutation: S286T
Source :
mol_id organism_scientific expression_system
1 Fusarium oxysporum  (taxid:5507) Escherichia coli  (taxid:562)
expression_system_strain: JM109(DE3)
expression_system_vector_type: COL E1
expression_system_vector: PRSET-C
expression_system_plasmid: PNORST
expression_system_gene: CYP55
Authors : Park, S.-Y., Shiro, Y.
Keywords : NITRIC OXIDE REDUCTASE, CYTOCHROME P450NOR, NO-LIGANDED, MUTAGENESIS(S286T), OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.70 Å )
Citation :

Proton delivery in NO reduction by fungal nitric-oxide reductase. Cryogenic crystallography, spectroscopy, and kinetics of ferric-NO complexes of wild-type and mutant enzymes.

Shimizu, H.,Obayashi, E.,Gomi, Y.  et al.
(2000)  J.Biol.Chem.  275 : 4816 - 4826

PubMed: 10671516
DOI: 10.1074/jbc.275.7.4816

Reaction

Chain : A
UniProt : P23295 (NOR_FUSOX)
Reaction: EC: Evidence:
Physiological Direction:
H2O + NADP(+) + nitrous oxide = H(+) + NADPH + 2 nitric oxide 1.7.1.14 PubMed:10671516, PubMed:15313618, PubMed:2040619, PubMed:7829493
-
H2O + NAD(+) + nitrous oxide = H(+) + NADH + 2 nitric oxide 1.7.1.14 PubMed:10671516, PubMed:15313618, PubMed:2040619, PubMed:7829493
-