Brand  (β version)

PDB ID: 1CJK

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Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
FOK Forskolin PoSSuM
GSP 5'-guanosine-diphosphate-monothiophosphate PoSSuM
MES 2-(N-morpholino)-ethanesulfonic acid PoSSuM
MG Magnesium ion PoSSuM
MN Manganese (II) ion PoSSuM
TAT Adenosine-5'-rp-alpha-thio-triphosphate PoSSuM
Non-standard Residues
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Glycosylation
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Modification
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Structure summary

Code : 1CJK   PDBj   RCSB PDB   PDBe
Header : LYASE/LYASE/SIGNALING PROTEIN
Title : COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH ADENOSINE 5'-(ALPHA THIO)-TRIPHOSPHATE (RP), MG, AND MN
Release Data : 1999-08-31
Compound :
mol_id molecule chains synonym
1 ADENYLATE CYCLASE, TYPE V A PROTEIN VC1
ec: 4.6.1.1
fragment: C1A DOMAIN OF ADENYLYL CYCLASE
mutation: V476M
mol_id molecule chains synonym
2 ADENYLATE CYCLASE, TYPE II B PROTEIN IIC2, ATP PYROPHOSPHATE-LYASE, ADENYLYL CYCLASE
ec: 4.6.1.1
fragment: C2A DOMAIN OF ADENYLYL CYCLASE
mol_id molecule chains synonym
3 GUANINE NUCLEOTIDE-BINDING PROTEIN G(S) C ALPHA SUBUNIT OF ADENYLATE CYCLASE-STIMULATING G ALPHA PROTEIN, GS-ALPHA, PROTEIN GSA
fragment: TRYPSINIZED FRAGMENT
mutation: N-TERMINAL HEXAHISTIDINE TAG
Source :
mol_id organism_scientific organism_common expression_system
1 Canis lupus familiaris  (taxid:9615) Dog Escherichia coli BL21(DE3)  (taxid:469008)
strain: familiaris
tissue: CARDIAC MUSCLE
cellular_location: CYTOPLASM
gene: ADENYLYL CYCLASE TYPE V
expression_system_strain: BL21
expression_system_variant: DE3
expression_system_vector_type: MULTI-COPY PLASMID
expression_system_plasmid: PQE60-H6-VC1(580)
mol_id organism_scientific organism_common expression_system
2 Rattus norvegicus  (taxid:10116) Norway rat Escherichia coli BL21(DE3)  (taxid:469008)
tissue: BRAIN
cellular_location: CYTOPLASM
gene: ADENYLYL CYCLASE TYPE II
expression_system_strain: BL21
expression_system_variant: DE3
expression_system_vector_type: MULTI-COPY PLASMID
expression_system_plasmid: PQE60-ARGC-IIC2
mol_id organism_scientific organism_common expression_system
3 Bos taurus  (taxid:9913) Cattle Escherichia coli BL21(DE3)  (taxid:469008)
cellular_location: CYTOPLASM
gene: GNAS
expression_system_strain: BL21
expression_system_variant: DE3
expression_system_vector_type: MULTI-COPY PLASMID
expression_system_plasmid: PQE60-GSALPHA-H
expression_system_gene: GNAS
Authors : Tesmer, J.J.G., Sprang, S.R.
Keywords : COMPLEX (LYASE-HYDROLASE), HYDROLASE, SIGNAL TRANSDUCING PROTEIN, CYCLASE, EFFECTOR ENZYME, LYASE-LYASE-SIGNALING PROTEIN COMPLEX
Exp. method : X-RAY DIFFRACTION ( 3.00 Å )
Citation :

Two-metal-Ion catalysis in adenylyl cyclase.

Tesmer, J.J.,Sunahara, R.K.,Johnson, R.A.  et al.
(1999)  Science  285 : 756 - 760

PubMed: 10427002
DOI: 10.1126/science.285.5428.756

Crystal structure of the catalytic domains of adenylyl cyclase in a complex with Gsalpha.GTPgammaS.

Tesmer, J.J.,Sunahara, R.K.,Gilman, A.G.  et al.
(1997)  Science  278 : 1907 - 1916

PubMed: 9417641
DOI: 10.1126/science.278.5345.1907

Reaction

Chain : A
UniProt : P30803 (ADCY5_CANLF)
Reaction: EC: Evidence:
Physiological Direction:
ATP = 3',5'-cyclic AMP + diphosphate 4.6.1.1 PubMed:10427002, PubMed:11087399, PubMed:15591060, PubMed:1618857, PubMed:16766715, PubMed:19243146, PubMed:8119955, PubMed:8428899, PubMed:9748257
-
Cofactor: Evidence: Note:
Mn(2+) ECO:0000269 | PubMed:11087399
ECO:0000269 | PubMed:16766715
ECO:0000269 | PubMed:19243146
ECO:0000269 | PubMed:11087399
ECO:0000269 | PubMed:16766715
Binds 2 magnesium ions per subunit (PubMed:16766715, PubMed:19243146). Is also active with manganese (in vitro) (PubMed:11087399, PubMed:16766715).
Chain : B
UniProt : P26769 (ADCY2_RAT)
Reaction: EC: Evidence:
Physiological Direction:
ATP = 3',5'-cyclic AMP + diphosphate 4.6.1.1 PubMed:10427002, PubMed:11087399, PubMed:15591060, PubMed:16766715, PubMed:1719547, PubMed:19243146, PubMed:21596131, PubMed:22906005, PubMed:24363043, PubMed:7761832
-
Cofactor: Evidence: Note:
Mn(2+) ECO:0000269 | PubMed:10427002
ECO:0000269 | PubMed:11087399
ECO:0000269 | PubMed:16766715
ECO:0000269 | PubMed:1719547
ECO:0000269 | PubMed:19243146
ECO:0000269 | PubMed:7761832
ECO:0000269 | PubMed:10427002
ECO:0000269 | PubMed:11087399
ECO:0000269 | PubMed:16766715
Binds 2 magnesium ions per subunit. Is also active with manganese (in vitro).
Chain : C
UniProt : P04896 (GNAS2_BOVIN)
Reaction: EC: Evidence:
Physiological Direction:
GTP + H2O = GDP + phosphate + H(+) - UniProtKB:P63092
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