Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

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Ligands
Code Name Link
CB3 10-propargyl-5,8-dideazafolic acid
UMP 2'-deoxyuridine 5'-monophosphate
Code : 1CI7
Header : TRANSFERASE
Title : TERNARY COMPLEX OF THYMIDYLATE SYNTHASE FROM PNEUMOCYSTIS CARINII
Release Data : 2000-04-10
Compound :
mol_id molecule chains
1 PROTEIN (THYMIDYLATE SYNTHASE) A,B
ec: 2.1.1.45
other_details: COVALENT BOND BETWEEN C173 AND DUMP IN MONOMER A
Source :
mol_id organism_scientific expression_system
1 Pneumocystis carinii  (taxid:4754) Escherichia coli  (taxid:562)
cellular_location: CYTOPLASM
expression_system_strain: CHI2913
expression_system_cellular_location: CYTOPLASM
expression_system_vector_type: PLASMID
expression_system_plasmid: PUETS-1.8
Authors : Anderson, A.C., O'Neil, R.H., Delano, W.L., Stroud, R.M.
Keywords : METHYLTRANSFERASE, NUCLEOTIDE BIOSYNTHESIS, HALF-SITES REACTIVITY, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.6 Å )
Citation :

The structural mechanism for half-the-sites reactivity in an enzyme, thymidylate synthase, involves a relay of changes between subunits.

Anderson, A.C.,O'Neil, R.H.,DeLano, W.L.  et al.
(1999)  Biochemistry  38 : 13829 - 13836

PubMed: 10529228
DOI: 10.1021/bi991610i

Chain : A, B
UniProt : P13100 (TYSY_PNECA)
Reaction : 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.