Brand  (β version)

PDB ID: 1CH8

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Ligands
Code Name Style Show Link
NO3 Nitrate ion PoSSuM
MG Magnesium ion PoSSuM
GPX Guanosine 5'-diphosphate 2':3'-cyclic monophosphate PoSSuM
HDA Hadacidin PoSSuM
IMP Inosinic acid PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1CH8   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : STRUCTURE OF ADENYLOSUCCINATE SYNTHETASE FROM E. COLI COMPLEXED WITH A STRINGENT EFFECTOR, PPG2':3'P
Release Data : 1999-12-29
Compound :
mol_id molecule chains synonym
1 PROTEIN (ADENYLOSUCCINATE SYNTHETASE) A AMPSASE
ec: 6.3.4.4
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K-12
expression_system_strain: PUR A- STRAIN H1238
expression_system_plasmid: PTRC99A
Authors : Hou, Z., Cashel, M., Fromm, H.J., Honzatko, R.B.
Keywords : LIGASE, ADENYLOSUCCINATE SYNTHETASE, GTP-HYDROLYSING ENZYMES, PURINE 2 NUCLEOTIDE BIOSYNTHESIS, PPGPP, PPG2':3'P, STRINGENT EFFECTOR
Exp. method : X-RAY DIFFRACTION ( 2.5 Å )
Citation :

Effectors of the stringent response target the active site of Escherichia coli adenylosuccinate synthetase.

Hou, Z.,Cashel, M.,Fromm, H.J.  et al.
(1999)  J.Biol.Chem.  274 : 17505 - 17510

PubMed: 10364182
DOI: 10.1074/jbc.274.25.17505

Reaction

Chain : A
UniProt : P0A7D4 (PURA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+) 6.3.4.4 HAMAP-Rule:MF_00011
-
Cofactor: Evidence: Note:
Mg(2+) - Binds 1 Mg(2+) ion per subunit.