Brand  (β version)

PDB ID: 1CD2

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Ligands
Code Name Style Show Link
FOL Folic acid PoSSuM
NAP Nadp nicotinamide-adenine-dinucleotide phosphate PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1CD2   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : LIGAND INDUCED CONFORMATIONAL CHANGES IN THE CRYSTAL STRUCTURES OF PNEUMOCYSTIS CARINII DIHYDROFOLATE REDUCTASE COMPLEXES WITH FOLATE AND NADP+
Release Data : 2000-03-06
Compound :
mol_id molecule chains synonym
1 DIHYDROFOLATE REDUCTASE A DHFR
ec: 1.5.1.3
other_details: COMPLEXED WITH NADP+ AND FOLATE
Source :
mol_id organism_scientific expression_system
1 Pneumocystis carinii  (taxid:4754) Escherichia coli  (taxid:562)
expression_system_plasmid: PT7-7
expression_system_gene: C-DNA P.CARINII DHFR
Authors : Cody, V., Galitsky, N., Luft, J.R., Pangborn, W., Blakley, R.L., Gangjee, A.
Keywords : OXIDOREDUCTASE, ONE-CARBON METABOLISM
Exp. method : X-RAY DIFFRACTION ( 2.200 Å )
Citation :

Ligand-induced conformational changes in the crystal structures of Pneumocystis carinii dihydrofolate reductase complexes with folate and NADP+.

Cody, V.,Galitsky, N.,Rak, D.  et al.
(1999)  Biochemistry  38 : 4303 - 4312

PubMed: 10194348
DOI: 10.1021/bi982728m

Comparison of ternary complexes of Pneumocystis carinii and wild-type human dihydrofolate reductase with coenzyme NADPH and a novel classical antitumor furo[2,3-d]pyrimidine antifolate.

Cody, V.,Galitsky, N.,Luft, J.R.  et al.
(1997)  Acta Crystallogr.,Sect.D  53 : 638 - 649

PubMed: 15299851
DOI: 10.1107/S090744499700509X

Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers.

Lewis, W.S.,Cody, V.,Galitsky, N.  et al.
(1995)  J.Biol.Chem.  270 : 5057 - 5064

PubMed: 7890613
DOI: 10.1074/jbc.270.10.5057

Methotrexate-resistant variants of human dihydrofolate reductase. Effects of Phe31 substitutions.

Chunduru, S.K.,Cody, V.,Luft, J.R.  et al.
(1994)  J.Biol.Chem.  269 : 9547 - 9555

PubMed: 8144541

Crystal structure determination at 2.3 A of recombinant human dihydrofolate reductase ternary complex with NADPH and methotrexate-gamma-tetrazole.

Cody, V.,Luft, J.R.,Ciszak, E.  et al.
(1992)  Anti-Cancer Drug Des.  7 : 483 - 491

PubMed: 1285840

Reaction

Chain : A
UniProt : P16184 (DYR_PNECA)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH 1.5.1.3 PROSITE- ProRule:PRU00660
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