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Ligands
Code Name Style Show Link
STU Staurosporine
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Code : 1BYG   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : KINASE DOMAIN OF HUMAN C-TERMINAL SRC KINASE (CSK) IN COMPLEX WITH INHIBITOR STAUROSPORINE
Release Data : 1999-10-14
Compound :
mol_id molecule chains synonym
1 PROTEIN (C-TERMINAL SRC KINASE) A CSK
ec: 2.7.1.112
fragment: KINASE DOMAIN
other_details: THE C-TERMINAL HISTIDINE TAG WAS NOT OBSERVED IN THE DENSITY AND IS NOT PRESENT IN THE SEQRES.
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
organ: LUNG
cell: GRANULOCYTE
expression_system_strain: DH5A
expression_system_vector_type: PLASMID
expression_system_vector: PQE60
expression_system_plasmid: PQE60-CSK
expression_system_gene: CSK
other_details: THE CDNA FOR THE CATALYTIC DOMAIN OF CSK WAS ISOLATED BY POLYMERASE CHAIN REACTION FROM GRANULOCYTE CDNA OBTAINED FROM HUMAN LUNG TISSUE. A HEXAHISTIDINE TAG WAS ENGINEERED AT THE C-TERMINUS TO AID PURIFICATION.
Authors : Antson, A.A., Lamers, M.B.A.C., Scott, R.K., Williams, D.H., Hubbard, R.E.
Keywords : PROTEIN KINASE, C-TERMINAL SRC KINASE, PHOSPHORYLATION, STAUROSPORINE, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.4 Å )
Citation :

Structure of the protein tyrosine kinase domain of C-terminal Src kinase (CSK) in complex with staurosporine.

Lamers, M.B.,Antson, A.A.,Hubbard, R.E.  et al.
(1999)  J.Mol.Biol.  285 : 713 - 725

PubMed: 9878439
DOI: 10.1006/jmbi.1998.2369

Chain : A
UniProt : P41240 (CSK_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl- [protein] 2.7.10.2 PubMed:1639064, PubMed:7683130, PubMed:9281320
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