Brand  (β version)

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hetatm:

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information
centroid:
interaction residue:

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Ligands
Code Name Link
NI Nickel (II) ion
SO4 Sulfate ion
Code : 1BS7   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : PEPTIDE DEFORMYLASE AS NI2+ CONTAINING FORM
Release Data : 1999-08-27
Compound :
mol_id molecule chains synonym
1 PROTEIN (PEPTIDE DEFORMYLASE) A,B,C PDF
ec: 3.5.1.31
other_details: PDF PROTEIN FROM ESCHERICHIA COLI IS CRYSTALLIZED AS NI2+ CONTAINING FORM
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
strain: JM109
cellular_location: CYTOPLASMA
gene: DEF
expression_system_strain: JM109
expression_system_vector_type: PLASMID
expression_system_vector: PKK223-3 (PHARMACIA)
expression_system_plasmid: P925C
expression_system_gene: DEF
Authors : Becker, A., Schlichting, I., Kabsch, W., Groche, D., Schultz, S., Wagner, A.F.V.
Keywords : HYDROLASE, IRON METALLOPROTEASE; PROTEIN SYNTHESIS
Exp. method : X-RAY DIFFRACTION ( 2.5 Å )
Citation :

Structure of peptide deformylase and identification of the substrate binding site.

Becker, A.,Schlichting, I.,Kabsch, W.  et al.
(1998)  J.Biol.Chem.  273 : 11413 - 11416

PubMed: 9565550
DOI: 10.1074/jbc.273.19.11413

Iron Center, Substrate Recognition and Mechanism of Peptide Deformylase

Becker, A.,Schlichting, I.,Kabsch, W.  et al.
(1998)  Nat.Struct.Biol.  5 : 1053

Isolation and Crystallization of Functionally Competent Escherichia Coli Peptide Deformylase Forms Containing Either Iron or Nickel in the Active Site

Groche, D.,Becker, A.,Schlichting, I.  et al.
(1998)  Biochem.Biophys.Res.Commun.  246 : 342

Chain : A, B, C
UniProt : P0A6K3 (DEF_ECOLI)
Reaction : Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide.