Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
CB3 10-propargyl-5,8-dideazafolic acid
UMP 2'-deoxyuridine 5'-monophosphate
Modified Residues
Code Name Link
CXM N-carboxymethionine
Code : 1BQ1
Header : TRANSFERASE
Title : E. COLI THYMIDYLATE SYNTHASE MUTANT N177A IN COMPLEX WITH CB3717 AND 2'-DEOXYURIDINE 5'-MONOPHOSPHATE (DUMP)
Release Data : 1999-05-18
Compound :
mol_id molecule chains
1 THYMIDYLATE SYNTHASE A,B
ec: 2.1.1.45
mutation: N177A
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
cell_line: X2913
gene: THYA
expression_system_vector: BLUESCRIPT VECTOR
expression_system_plasmid: PTHYA-N177A
expression_system_gene: THYA
Authors : Reyes, C.L., Sage, C.R., Rutenber, E.E., Finer-Moore, J.S., Stroud, R.M.
Keywords : TRANSFERASE, ACTIVE SITE MUTANT, REACTION INTERMEDIATE
Exp. method : X-RAY DIFFRACTION ( 2.5 Å )
Citation :

Inactivity of N229A thymidylate synthase due to water-mediated effects: isolating a late stage in methyl transfer.

Reyes, C.L.,Sage, C.R.,Rutenber, E.E.  et al.
(1998)  J.Mol.Biol.  284 : 699 - 712

PubMed: 9826509
DOI: 10.1006/jmbi.1998.2205

Chain : A, B
UniProt : P0A884 (TYSY_ECOLI)
Reaction : 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.