Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
K Potassium ion
UMP 2'-deoxyuridine 5'-monophosphate
Code : 1BP6
Header : TRANSFERASE
Title : THYMIDYLATE SYNTHASE R23I, R179T DOUBLE MUTANT
Release Data : 1998-08-19
Compound :
mol_id molecule chains
1 PROTEIN (THYMIDYLATE SYNTHASE) A
ec: 2.1.1.45
mutation: R23I, R179T
Source :
mol_id organism_scientific expression_system
1 Lactobacillus casei  (taxid:1582) Escherichia coli  (taxid:562)
strain: E. COLI CHI-2913
expression_system_strain: CHI-2913
expression_system_plasmid: PKPTSD
other_details: SYNTHETIC GENE
Authors : Morse, R.J., Finer-Moore, J.S., Stroud, R.M.
Keywords : TRANSFERASE (METHYLTRANSFERASE), TRANSFERASE, METHYLTRANSFERASE, NUCLEOTIDE BIOSYNTHESIS
Exp. method : X-RAY DIFFRACTION ( 2.40 Å )
Citation :

Energetic contributions of four arginines to phosphate-binding in thymidylate synthase are more than additive and depend on optimization of "effective charge balance".

Morse, R.J.,Kawase, S.,Santi, D.V.  et al.
(2000)  Biochemistry  39 : 1011 - 1020

PubMed: 10653645
DOI: 10.1021/bi9918590

Stereochemistry of a Multistep(Slash)Bipartite Methyl Transfer Reaction: Thymidylate Synthase

Stroud, R.M.,Finer-Moore, J.S.
(1993)  Faseb J.  7 : 671

Refined Structures of Substrate-Bound and Phosphate-Bound Thymidylate Synthase from Lactobacillus Casei

Finer-Moore, J.S.,Fauman, E.B.,Foster, P.G.  et al.
(1993)  J.Mol.Biol.  232 : 1101

Atomic Structure of Thymidylate Synthase: Target for Rational Drug Design

Hardy, L.W.,Finer-Moore, J.S.,Montfort, W.R.  et al.
(1987)  Science  235 : 448

Chain : A
UniProt : P00469 (TYSY_LACCA)
Reaction : 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.