Brand  (β version)

color scheme of protein:

chain:

Modified Residues
Code Name Link
CSS S-mercaptocysteine
Code : 1BOI
Header : TRANSFERASE
Title : N-TERMINALLY TRUNCATED RHODANESE
Release Data : 1999-04-27
Compound :
mol_id molecule chains
1 RHODANESE A
ec: 2.8.1.1
fragment: DEL(1-7)
Source :
mol_id organism_scientific organism_common expression_system
1 Bos taurus  (taxid:9913) Cattle Escherichia coli BL21  (taxid:511693)
cell_line: 293
organ: LIVER
cellular_location: CYTOPLASM
expression_system_strain: BL21
expression_system_cellular_location: CYTOPLASM
expression_system_vector: PLASMID
expression_system_plasmid: PTTQ19
Authors : Gliubich, F., Berni, R., Cianci, M., Trevino, R.J., Horowitz, P.M., Zanotti, G.
Keywords : TRANSFERASE, RHODANESE, SULFURTRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.2 Å )
Citation :

NH2-terminal sequence truncation decreases the stability of bovine rhodanese, minimally perturbs its crystal structure, and enhances interaction with GroEL under native conditions.

Trevino, R.J.,Gliubich, F.,Berni, R.  et al.
(1999)  J.Biol.Chem.  274 : 13938 - 13947

PubMed: 10318804
DOI: 10.1074/jbc.274.20.13938

Structure of Sulfur-Substituted Rhodanese at 1.36 A Resolution

Gliubich, F.,Berni, R.,Colapietro, M.  et al.
(1998)  Acta Crystallogr.,Sect.D  54 : 481

Active Site Structural Features for Chemically Modified Forms of Rhodanese

Gliubich, F.,Gazerro, M.,Zanotti, G.  et al.
(1996)  J.Biol.Chem.  271 : 21054

The Structure of Bovine Liver Rhodanese. II. The Active Site in the Sulfur-Substituted and the Sulfur-Free Enzyme

Ploegman, J.H.,Drent, G.,Kalk, K.H.  et al.
(1979)  J.Mol.Biol.  127 : 149

Chain : A
UniProt : P00586 (THTR_BOVIN)
Reaction : Thiosulfate + cyanide = sulfite + thiocyanate.