Brand  (β version)

PDB ID: 1BEZ

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ACY Acetic acid PoSSuM
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Structure summary

Code : 1BEZ   PDBj   RCSB PDB   PDBe
Header : DEHALOGENASE
Title : HALOALKANE DEHALOGENASE MUTANT WITH TRP 175 REPLACED BY TYR AT PH 5
Release Data : 1998-11-11
Compound :
mol_id molecule chains
1 HALOALKANE DEHALOGENASE A
ec: 3.8.1.5
mutation: I2V, W175Y
other_details: WITH ACETIC ACID
Source :
mol_id organism_scientific expression_system
1 Xanthobacter autotrophicus  (taxid:280) Escherichia coli  (taxid:562)
strain: GJ10
Authors : Ridder, I.S., Vos, G.J., Rozeboom, H.J., Kalk, K.H., Dijkstra, B.W.
Keywords : DEHALOGENASE, ALPHA/BETA-HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.1 Å )
Citation :

Kinetic analysis and X-ray structure of haloalkane dehalogenase with a modified halide-binding site.

Krooshof, G.H.,Ridder, I.S.,Tepper, A.W.  et al.
(1998)  Biochemistry  37 : 15013 - 15023

PubMed: 9790663
DOI: 10.1021/bi9815187

Crystallographic Analysis of the Catalytic Mechanism of Haloalkane Dehalogenase

Verschueren, K.H.,Seljee, F.,Rozeboom, H.J.  et al.
(1993)  Nature  363 : 693

Refined X-Ray Structures of Haloalkane Dehalogenase at Ph 6.2 And Ph 8.2 And Implications for the Reaction Mechanism

Verschueren, K.H.,Franken, S.M.,Rozeboom, H.J.  et al.
(1993)  J.Mol.Biol.  232 : 856

Crystal Structure of Haloalkane Dehalogenase: An Enzyme to Detoxify Halogenated Alkanes

Franken, S.M.,Rozeboom, H.J.,Kalk, K.H.  et al.
(1991)  Embo J.  10 : 1297

Crystallization of Haloalkane Dehalogenase from Xanthobacter Autotrophicus Gj10

Rozeboom, H.J.,Kingma, J.,Janssen, D.B.  et al.
(1988)  J.Mol.Biol.  200 : 611

Reaction

Chain : A
UniProt : P22643 (DHLA_XANAU)
Reaction: EC: Evidence:
Physiological Direction:
1-haloalkane + H2O = a halide anion + a primary alcohol + H(+) 3.8.1.5 -
-
1,2-dichloroethane + H2O = 2-chloroethanol + chloride + H(+) 3.8.1.5 -
-